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Annals of Botany 87: 457-462, 2001
© 2001 Annals of Botany Company

Hybrid Identification in Clivia(Amaryllidaceae) using Chromosome Banding and Genomic In Situ Hybridization

Yidong Ran, Keith R. W. Hammett and Brian G. Murray+

School of Biological Sciences, The University of Auckland, Private Bag 92019, Auckland, New Zealand School of Biological Sciences, The University of Auckland, 488c Don Buck Road, Massey, Auckland 8, New Zealand

Received: 11 September 2000 ; Returned for revision: 2 November 2000 . Accepted: 11 December 2000

Giemsa C-banding and genomic in situ hybridization (GISH) were used to identify parental genomes in hybrids of Clivia(Amaryllidaceae). Of the three groups reputed to be hybrids, onlyC. cyrtanthiflora was shown to be of hybrid origin. The ‘German hybrids’ and ‘Belgian hybrids’ were both shown to be karyotypically and genomically similar to C. miniata, and are either selections or intraspecific hybrids of that species. Successful genome differentiation in F1hybrids by GISH required high stringency and high ratios of blocking DNA to probe. The spatial disposition of different genomes with C-band or GISH markers in the hybrids was investigated in two dimensions on the spread. In five artificially produced hybrids, either C-banding or GISH was used to locate the position of parental genomes in mitotic metaphase cells. In all cases there was a significant tendency for centromeres of the different parental genomes to occupy two distinct concentric domains on the metaphase plate. The presence or absence of centromeric heterochromatin was not correlated with genome disposition. Results show that chromosome analyses can be a useful way of identifying Clivia hybrids in their vegetative phase. Copyright 2001 Annals of Botany Company

Clivia, genomic in situ hybridization, cultivar origin, parental genome separation


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