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Annals of Botany 87: 599-603, 2001
© 2001 Annals of Botany Company

Thermal Dependence of the Apparent Kmof Glutathione Reductase from Three Wetland Grasses and Maize

Stephen M. Griffith+, Timothy G. Brewer and Jeffrey J. Steiner

United States Department of Agriculture, Agricultural Research Service, 3450 SW Campus Way, Corvallis, Oregon 97331, USA Department of Crop and Soil Science, Oregon State University, USA

Received: 17 August 2000 ; Returned for revision: 24 August 2000 . Accepted: 19 January 2001

The thermal dependence of enzyme kinetic parameters has been presented as an indicator of species’ thermal optima and tolerance limits. Previous studies suggest the relationship between temperature and the apparent Michaelis–Menten constant (Km) of an enzyme system can be used to predict whole plant success at specific temperatures. The apparent Kmfor glutathione reductase (EC 1.6.4.2; GR) (oxidized glutathione as substrate) extracted from leaves of American sloughgrass (Beckmannia syzigachne Steud.), tufted hairgrass (Deschampsia caespitosa L.), tall fescue (Festuca arundinaceae Schreb. ‘Titan’), and maize (Zea mays L.), was determined over a range of temperatures (1–40 °C). For all species, minimum apparent Kmfor GR was observed at 1 °C, and Kmvalues increased as temperature increased. The apparent Kmvalues differed among all species at the lower temperatures (1–15 °C), but were similar at higher temperatures. The enzyme from tufted hairgrass had the lowest apparent Kmat low temperatures (<15 °C), followed in increasing order by American sloughgrass, tall fescue and maize. Our experimental system failed to reproduce thermal kinetic window profiles similar to those reported elsewhere. With respect to the enzyme systems reported here, results suggest that these cool-season grasses can be ranked as more to less eurythermic within the temperature range from 1 to 15 °C. Copyright 0000

American sloughgrass, Beckmannia syzigachne Steud., tufted hairgrass, Deschampsia caespitosa L., tall fescue, Festuca arundinaceae Schreb. ‘Titan’, Zea mays L., plant competition, temperature stress, kinetics, Michaelis–Menten constant (Km), glutathione reductase


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 Agron. J.Home page
J. J. Steiner, T. G. Brewer, and S. M. Griffith
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Agron. J., September 1, 2001; 93(5): 1020 - 1027.
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