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Annals of Botany 89: 351-354, 2002
© 2002 Annals of Botany Company

Glufosinate-tolerant Tobacco Plants Directed by the Promoter of Adenylate Kinase Gene of Rice

HIROMITSU FUKUZAWA1, SATOSHI ARAI1, MAKI KAWAI-YAMADA2, AVIJIT DAS2, MICHITO TAGAWA1 and HIROFUMI UCHIMIYA*,2

1Department of Agricultural Chemistry, Shiraoka Research Station of Biological Science, Nissan Chemical Industries Ltd, 1470 Shiraoka, Minamisaitama, Saitama 349–02 and 2Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi 1–1-1, Bunkyo-ku, Tokyo 113, Japan

* For correspondence: Fax +81 3 58418466, e-mail uchimiya{at}iam.u-tokyo.ac.jp

Received: 30 July 2001; Returned for revision: 10 October 2001; Accepted: 21 November 2001.

ABSTRACT

A DNA clone containing the 5' part of the adenylate kinase (AK) gene was isolated from a rice genomic library, and its nucleotide sequence was determined. This clone consists of 5' upstream, five exons and four introns of the AK gene. All of the determined donor and receptor sites contained ‘GT’ and ‘AG’ consensus splice sequences. Transgenic tobacco plants harbouring a chimeric gene consisting of the 5' upstream sequence of the AK gene fused with the gene encoding phosphinothricin acetyl transferase were generated. They showed tolerance to glufosinate to a level four times higher than its commercial dose.

Key words: Adenylate kinase, gene promoter, glufosinate resistance, tobacco, rice.


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