Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Content Snapshot
Right arrow Content Select
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (10)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by POUND, L. M.
Right arrow Articles by SEDGLEY, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by POUND, L. M.
Right arrow Articles by SEDGLEY, M.
Agricola
Right arrow Articles by POUND, L. M.
Right arrow Articles by SEDGLEY, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Annals of Botany 89: 613-620, 2002
© 2002 Annals of Botany Company

Early Ovule Development Following Self- and Cross-pollinations in Eucalyptus globulus Labill. ssp. globulus

L. M. POUND1, M. A. B. WALLWORK1, B. M. POTTS2 and M. SEDGLEY*,1

1Department of Horticulture, Viticulture and Oenology, Adelaide University, Waite Campus, P.M.B. 1, Glen Osmond, South Australia 5064, Australia and 2Cooperative Research Centre for Sustainable Production Forestry, andSchool of Plant Science, University of Tasmania, G.P.O. 252–55, Hobart, Tasmania 7001, Australia

* For correspondence. E-mail margaret.sedgley{at}adelaide.edu.au

Received: 5 November 2001; Returned for revision: 19 December 2001; Accepted: 28 January 2002.

The study was conducted to identify the self-incompatibility mechanism in Eucalyptus globulus ssp. globulus. Controlled self- and cross-pollinations were conducted on individual flowers from three mature trees that had self-incompatibility levels of 76, 99·6 and 100 %. Flowers were harvested at 4, 6 and 8 weeks after pollination. Embryology was investigated by bright field microscopy on material harvested at 4 and 6 weeks after pollination. Fertilization had taken place at 4 weeks after pollination with zygotes and free nuclear endosperm visible. There was a greater proportion of healthy, fertilized ovules in the cross- compared with the self-pollination treatment, and approx. half the ovules examined from both pollen treatments were not fertilized or were degenerating. By 6 weeks after pollination a few zygotes were starting to divide. The number of healthy, fertilized ovules was still greater in the cross-pollination treatment, but the number of healthy fertilized ovules was lower in both treatments compared with 4 weeks after pollination, and many ovules were degenerating. Fertilized ovules were significantly larger than non-fertilized or degenerating ovules and this difference was detectable by eye at 6 and 8 weeks after pollination. The mechanism of self-incompatibility appears to have both late pre- and post-zygotic components.

Key words: Eucalyptus globulus ssp. globulus, Tasmanian blue gum, self-incompatibility, controlled pollination, bright field microscopy, embryology, fertilization, ovule, zygote.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.