Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Content Snapshot
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (7)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by GERSBACH, P. V.
Right arrow Articles by REDDY, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by GERSBACH, P. V.
Right arrow Articles by REDDY, N.
Agricola
Right arrow Articles by GERSBACH, P. V.
Right arrow Articles by REDDY, N.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Annals of Botany 90: 253-257, 2002
© 2002 Annals of Botany Company

Non-invasive Localization of Thymol Accumulation in Carum copticum (Apiaceae) Fruits by Chemical Shift Selective Magnetic Resonance Imaging

P. V. GERSBACH*,1 and N. REDDY2

1 Centre for Biostructural and Biomolecular Research, University of Western Sydney, Richmond, NSW 2753, Australia and 2 School of Science, Food and Horticulture, University of Western Sydney, Kingswood, NSW, Australia

* For correspondence. E-mail: p_gersbach{at}hotmail.com

Received: 4 February 2002; Returned for revision: 2 April 2002; Accepted: 10 May 2002

Magnetic resonance imaging was used to localize the site of essential oil accumulation in fruit of Carum copticum L. (Apiaceae). A chemical shift method is described that utilized the spectral properties of the aromatic monoterpene thymol, the major component of the essential oil, to image thymol selectively. The presence of essential oil secretory structures in the fruit and an essential oil containing a high proportion of thymol were confirmed with optical microscopy and gas chromatography-mass spectrometry, respectively. Selective imaging of whole C. copticum fruits showed that thymol accumulation was localized to the secretory structures (canals) situated in the fruit wall. The technique was considered non-invasive as the seeds used in the imaging experiments remained intact and viable.

Key words: Carum copticum, ajowan, nuclear magnetic resonance, magnetic resonance imaging, chemical shift imaging, histochemistry, essential oil, monoterpenoids, thymol.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.