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Annals of Botany 90: 269-278, 2002
© 2002 Annals of Botany Company

Structural and Ultrastructural Analysis of Solanum lycopersicoides Protoplasts during Diploid Plant Regeneration

ADAM TYLICKI1, WOJCIECH BURZA2, STEFAN MALEPSZY2, MARIOLA KULAWIEC4 and MIECZYSLAW KURAS*,3

1 Department of Animal Biochemistry, Institute of Biology, University of Bialystok, Swierkowa 20 B, 15-950 Bialystok, Poland, 2 Department of Plant Genetics, Breeding and Biotechnology, SGGW, Nowoursynowska 166, 02-787 Warsaw, Poland, 3 Department of Plant Morphogenesis, Warsaw University, Miecznikowa 1, 02-097 Warsaw, Poland and 4 Department of Cell Biology, Maria Curie-Sklodowska University, Akademicka 19, 02-032 Lublin, Poland

* For correspondence. E-mail kuras{at}biol.uw.edu.pl

Received: 21 March 2002; Accepted: 15 May 2002

Light, fluorescence and electron microscopy were used to analyse the structural properties of protoplasts obtained from established suspension culture of Solanum lycopersicoides Dun. composed of meristematic cell aggregates. Four types of protoplasts were distinguished immediately after isolation: (1) mononuclear; (2) polynuclear, (3) anuclear and (4) homogeneous protoplasts. Only mononuclear protoplasts were capable of complete cell wall regeneration and mitotic division. Other types of protoplasts were eliminated during culture. Three phases were distinguished in the developing protoplast culture: (1) the elimination phase during which protoplasts damaged during isolation underwent complete degradation; (2) a phase of intense division during which both mitotic cell division and amitotic nuclear division took place; and (3) a stabilization phase leading to the formation of suspension culture. The cell suspension culture obtained from protoplasts was capable of regenerating diploid plants.

Key words: Solanum lycopersicoides, protoplast culture, structure, ultrastructure, plant regeneration, genetic stability.


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