Annals of Botany 91: 279-290, 2003
© 2003 Annals of Botany Company
Dynamic Aspects of Alcoholic Fermentation of Rice Seedlings in Response to Anaerobiosis and to Complete Submergence: Relationship to Submergence Tolerance
1 Life Science Trace Gas Exchange Facility, Department of Molecular and Laser Physics, University of Nijmegen, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands 2 Centre of Advanced Studies, Department of Crop Physiology, Narendra Deva University of Agriculture & Technology, Kumarganj, 224229, Faizabad, India and 3 School of Biological Sciences, University of Bristol, Woodland Road, Bristol BS8 1UG, UK
* For correspondence. Tel +31-24-3653029, Fax +31-24-3653311, e-mail iulia{at}sci.kun.nl
Received: 15 June 2001; Returned for revision: 8 February 2002; Accepted: 13 June 2002
Rice plants are severely damaged by complete submergence. This is a problem in rice farming and could be the result, in part, of tissue anoxia imposed by a reduced availability of oxygen. To investigate this possibility we monitored alcoholic fermentation products as markers for tissue anaerobiosis using sensitive laser-based spectroscopy able to sense ethanol and acetaldehyde down to 3 nl l–1 and 0·1 nl l–1, respectively. Acetaldehyde emission began within 0·5 h of imposing an oxygen-free gas phase environment followed closely by ethanol. As treatment progressed, ethanol output increased and came to exceed acetaldehyde emission as this stabilized considerably after approx. 3 h. On re-entry of air, a sharp post-anaerobic peak of acetaldehyde production was observed. This was found to be diagnostic of a preceding anoxic episode of 0·5 h or more. When anaerobiosis was lengthened by up to 14 h, the size of the post-anaerobic acetaldehyde outburst increased. After de-submergence from oxygen-free water, a similarly strong but slower post-anaerobic acetaldehyde upsurge was seen, which was accompanied by an increase in ethanol emission. Light almost, but not completely, eliminated fermentation in anaerobic surroundings and also the post-anaerobic or post-submergence peaks in acetaldehyde production. All photosynthetically generated oxygen was consumed within the plant. There was no substantial difference in acetaldehyde and ethanol output between FR13A and the less submergence-tolerant line CT6241 under any submergence treatment. In some circumstances, submergence damaged CT6241 more than FR13A even in the absence of vigorous fermentation. We conclude that oxygen deprivation may not always determine the extent of damage caused to rice plants by submergence under natural conditions.
Key words: Rice seedlings, Oryza sativa L., anaerobiosis, complete submergence, post-anoxia, acetaldehyde, ethanol, carbon dioxide, oxygen, trace gas detection.
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