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Annals of Botany 91: 465-471, 2003
© 2003 Annals of Botany Company

Inhibition of the Indole-3-acetic acid-induced Epinastic Curvature in Tobacco Leaf Strips by 2,4-Dichlorophenoxyacetic Acid

NAKAKO KAWANO1, TOMONORI KAWANO1,2 and FREDERIC LAPEYRIE*,1

1 Unité Mixte de Recherche INRA-UHP Interactions Arbres/Micro-organismes, Institut National de la Recherche Agronomique, F-54280 Champenoux, France and 2 Department of Biological Science, Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Japan

* For correspondence. Fax +33 383 394069, e-mail lapeyrie{at}nancy.inra.fr

Received: 4 September 2002; Returned for revision: 18 October 2002; Accepted: 27 November 2002

It has been reported that auxin induces an epinastic growth response in plant leaf tissues. Leaf strips of tobacco (Nicotiana tabacum L. ‘Bright Yellow 2’) were used to study the effects of indole-3-acetic acid (IAA), the principal form of auxin in higher plants, and a synthetic auxin, 2,4-dichlorophenoxyacetic acid (2,4-D), on epinastic leaf curvature. Incubation of leaf strips with 10 µM IAA resulted in a marked epinastic curvature response. Unexpectedly, 2,4-D showed only a weak IAA-like activity in inducing epinasty. Interestingly, the presence of 2,4-D resulted in inhibition of the IAA-dependent epinastic curvature. In vivo Lineweaver–Burk kinetic analysis clearly indicated that the interaction between IAA and 2,4-D reported here is not a result of competitive inhibition. Using kinetic analysis, it was not possible to determine whether the mode of interaction between IAA and 2,4-D was non-competitive or uncompetitive. 2,4-D inhibits the IAA-dependent epinasty via complex and as yet unidentified mechanisms.

Key words: Auxin, bioassay, 2,4-D, epinasty, IAA, inhibition, leaf curvature, Nicotiana tabacum, tobacco.


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