Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Content Snapshot
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (12)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by LEXA, M.
Right arrow Articles by BRZOBOHATY, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by LEXA, M.
Right arrow Articles by BRZOBOHATY, B.
Agricola
Right arrow Articles by LEXA, M.
Right arrow Articles by BRZOBOHATY, B.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Annals of Botany 91: 585-597, 2003
© 2003 Annals of Botany Company

Dynamics of Endogenous Cytokinin Pools in Tobacco Seedlings: a Modelling Approach

MATEJ LEXA1, TODOR GENKOV1, JIRÍ MALBECK2, IVANA MACHÁCKOVÁ2 and BRETISLAV BRZOBOHATY1,3

1 Masaryk University Brno, Department of Functional Genomics and Proteomics, Kotlárská 2, 611 37 Brno, Czech Republic, 2 Institute of Experimental Botany of the Academy of Sciences of the Czech Republic, Rozvojová 135, 165 02 Prague, Czech Republic and 3 Institute of Biophysics of the Academy of Sciences of the Czech Republic, Královopolská 135, 612 65 Brno, Czech Republic

* For correspondence. Fax: +420-541129372, e-mail: m-lexa{at}sci.muni.cz

Received: 13 November 2002; Returned for revision: 19 December 2002; Accepted: 7 January 2003

Recent advances in cytokinin analysis have made it possible to measure the content of 22 cytokinin metabolites in the tissue of developing tobacco seedlings. Individual types of cytokinins in plants are interconverted to their respective forms by several enzymatic activities (5'-AMP-isopentenyltransferase, adenosine nucleosidase, 5'-nucleotidase, adenosine phosphorylase, adenosine kinase, trans-hydroxylase, zeatin reductase, ß-glucosidase, O-glucosyl transferase, N-glucosyl transferase, cytokinin oxidase). This paper reports modelling and measuring of the dynamics of endogenous cytokinins in tobacco plants grown on media supplemented with isopentenyl adenine (IP), zeatin (Z) and dihydrozeatin riboside (DHZR). Differences in phenotypes generated by the three cytokinins are shown and discussed, and the assumption that substrate concentration drives enzyme kinetics underpinned the construction of a simple mathematical model of cytokinin metabolism in developing seedlings. The model was tested on data obtained from liquid chromatography/tandem mass spectrometry cytokinin measurements on tobacco seedlings grown on Murashige and Skoog agar nutrient medium, and on plants grown in the presence of IP, Z and DHZR. A close match was found between measured and simulated data, especially after a series of iterative parameter searches, in which the parameters were set to obtain the best fit with one of the data sets.

Key words: Endogenous cytokinins, zeatin, isopentenyladenine, dihydrozeatin, metabolism, mathematical simulation model, Nicotiana tabacum L., tobacco.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
G. Lochmanova, Z. Zdrahal, H. Konecna, S. Koukalova, J. Malbeck, P. Soucek, M. Valkova, N. S. Kiran, and B. Brzobohaty
Cytokinin-induced photomorphogenesis in dark-grown Arabidopsis: a proteomic analysis
J. Exp. Bot., October 1, 2008; 59(13): 3705 - 3719.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
A. Kuderova, I. Urbankova, M. Valkova, J. Malbeck, B. Brzobohaty, D. Nemethova, and J. Hejatko
Effects of Conditional IPT-Dependent Cytokinin Overproduction on Root Architecture of Arabidopsis Seedlings
Plant Cell Physiol., April 1, 2008; 49(4): 570 - 582.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
N. S. Kiran, L. Polanska, R. Fohlerova, P. Mazura, M. Valkova, M. Smeral, J. Zouhar, J. Malbeck, P. I. Dobrev, I. Machackova, et al.
Ectopic over-expression of the maize {beta}-glucosidase Zm-p60.1 perturbs cytokinin homeostasis in transgenic tobacco
J. Exp. Bot., March 1, 2006; 57(4): 985 - 996.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. Novakova, V. Motyka, P. I. Dobrev, J. Malbeck, A. Gaudinova, and R. Vankova
Diurnal variation of cytokinin, auxin and abscisic acid levels in tobacco leaves
J. Exp. Bot., November 1, 2005; 56(421): 2877 - 2883.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.