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Annals of Botany 91: 921-926, 2003
© 2003 Annals of Botany Company

Karyotype Analysis of Four Vicia Species using In Situ Hybridization with Repetitive Sequences

ALICE NAVRÁTILOVÁ1, PAVEL NEUMANN1 and JIRÍ MACAS1

1 Institute of Plant Molecular Biology, Laboratory of Molecular Cytogenetics, Branisovská 31, Ceské Budejovice, CZ-37005, Czech Republic

* For correspondence. Fax +420 385310356, e-mail macas{at}umbr.cas.cz

Received: 9 December 2002; Returned for revision: 17 February 2003; Accepted: 5 March 2003

Mitotic chromosomes of four Vicia species (V. sativa, V. grandiflora, V. pannonica and V. narbonensis) were subjected to in situ hybridization with probes derived from conserved plant repetitive DNA sequences (18S–25S and 5S rDNA, telomeres) and genus-specific satellite repeats (VicTR-A and VicTR-B). Numbers and positions of hybridization signals provided cytogenetic landmarks suitable for unambiguous identification of all chromosomes, and establishment of the karyotypes. The VicTR-A and -B sequences, in particular, produced highly informative banding patterns that alone were sufficient for discrimination of all chromosomes. However, these patterns were not conserved among species and thus could not be employed for identification of homologous chromosomes. This fact, together with observed variations in positions and numbers of rDNA loci, suggests considerable divergence between karyotypes of the species studied.

Key words: Vicia, in situ hybridization, PRINS, rRNA genes, satellite DNA, repetitive DNA, karyotype evolution.


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