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Annals of Botany 92: 145-151, 2003
© 2003 Annals of Botany Company

Origin and Development In Vitro of Shoot Buds and Somatic Embryos from Intact Roots of Helianthus annuus x H. tuberosus

M. FAMBRINI1, G. CIONINI2, A. CONTI1, V. MICHELOTTI1 and C. PUGLIESI*,1

1 Dipartimento di Biologia delle Piante Agrarie, Sezione di Genetica, Via Matteotti 1B, 56124 Pisa, Italy and 2 Istituto di Biologia e Biotecnologie Agrarie (IBBA)—CNR, Area della Ricerca San Cataldo, Via Moruzzi 1, 56124 Pisa, Italy

* For correspondence. Fax +39 (0) 50 576750, e-mail cpuglies{at}agr.unipi.it

Received: 15 November 2002; Returned for revision: 24 February 2003; Accepted: 8 April 2003

ABSTRACT

A variant clone of the tetraploid (2n = 4x = 68) interspecific hybrid Helianthus annuus x H. tuberosus derived by in vitro tissue culture showed a deviation from the usual pattern of organization of the plant body. This variant developed shoot-like structures and somatic embryos from intact adventitious roots of in vitro-grown plantlets. The morphogenetic structures were not normally able to differentiate complete plants. They did show cellular proliferation with the inception of additional secondary embryos, leaf-like structures and unorganized masses of callus. Nevertheless, some ectopic structures isolated from roots and transferred onto fresh basal medium without growth regulators were able to produce plantlets that exhibited the same phenotype as the original clone. Histological analyses demonstrate that they originate from cortical cells in association with the development of lateral root primordia.

Key words: Helianthus annuus x H. tuberosus, interspecific hybrid, regeneration in vitro, root buds, somatic embryogenesis, totipotency.


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