Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Content Snapshot
Right arrow Content Select
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (19)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by NOIROT, M.
Right arrow Articles by HAMON, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by NOIROT, M.
Right arrow Articles by HAMON, S.
Agricola
Right arrow Articles by NOIROT, M.
Right arrow Articles by HAMON, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Annals of Botany 92: 259-264, 2003
© 2003 Annals of Botany Company

Effects of Caffeine and Chlorogenic Acid on Propidium Iodide Accessibility to DNA: Consequences on Genome Size Evaluation in Coffee Tree

M. NOIROT*,1, P. BARRE3, C. DUPERRAY2, J. LOUARN4 and S. HAMON1

1 IRD BP5045, 34032 Montpellier Cedex, France, 2 INSERM, Unité 291, 99 rue du P. Villa, 34090 Montpellier, France, 3 INRA-SAPF, 86600 Lusignan, Franceand 4 IRD, BP 434, Man, Côte d’Ivoire

* For correspondence. E-mail noirot{at}mpl.ird.fr

Received: 30 June 2002;; Returned for revision: 30 January 2003. Accepted: 13 May 2003

Estimates of genome size using flow cytometry can be biased by the presence of cytosolic compounds, leading to pseudo-intraspecific variation in genome size. Two important compounds present in coffee trees—caffeine and chlorogenic acid—modify accessibility of the dye propidium iodide to Petunia DNA, a species used as internal standard in our genome size evaluation. These compounds could be responsible for intraspecific variation in genome size since their contents vary between trees. They could also be implicated in environmental variations in genome size, such as those revealed when comparing the results of evaluations carried out on different dates on several genotypes.

Key words: Coffea, nuclear DNA content, flow cytometry, dye accessibility, caffeine, chlorogenic acids.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 ANN BOT (LOND)Home page
J. Greilhuber
Cytochemistry and C-values: The Less-well-known World of Nuclear DNA Amounts
Ann. Bot., April 1, 2008; 101(6): 791 - 804.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
M. D. Bennett, H. J. Price, and J. S. Johnston
Anthocyanin Inhibits Propidium Iodide DNA Fluorescence in Euphorbia pulcherrima: Implications for Genome Size Variation and Flow Cytometry
Ann. Bot., April 1, 2008; 101(6): 777 - 790.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
J. LOUREIRO, E. RODRIGUEZ, J. DOLEZEL, and C. SANTOS
Comparison of Four Nuclear Isolation Buffers for Plant DNA Flow Cytometry
Ann. Bot., September 1, 2006; 98(3): 679 - 689.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
J. LOUREIRO, E. RODRIGUEZ, J. DOLEZEL, and C. SANTOS
Flow Cytometric and Microscopic Analysis of the Effect of Tannic Acid on Plant Nuclei and Estimation of DNA Content
Ann. Bot., September 1, 2006; 98(3): 515 - 527.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
P. SMARDA and P. BURES
Intraspecific DNA Content Variability in Festuca pallens on Different Geographical Scales and Ploidy Levels
Ann. Bot., September 1, 2006; 98(3): 665 - 678.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
B. HENDRIX and J. McD. STEWART
Estimation of the Nuclear DNA Content of Gossypium Species
Ann. Bot., April 1, 2005; 95(5): 789 - 797.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
M. D. BENNETT and I. J. LEITCH
Plant Genome Size Research: A Field In Focus
Ann. Bot., January 1, 2005; 95(1): 1 - 6.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
J. DOLEZEL and J. BARTOS
Plant DNA Flow Cytometry and Estimation of Nuclear Genome Size
Ann. Bot., January 1, 2005; 95(1): 99 - 110.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
M. NOIROT, P. BARRE, C. DUPERRAY, S. HAMON, and A. DE KOCHKO
Investigation on the Causes of Stoichiometric Error in Genome Size Estimation Using Heat Experiments: Consequences on Data Interpretation
Ann. Bot., January 1, 2005; 95(1): 111 - 118.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
M. NOIROT, V. PONCET, P. BARRE, P. HAMON, S. HAMON, and A. DE KOCHKO
Genome Size Variations in Diploid African Coffea Species
Ann. Bot., November 1, 2003; 92(5): 709 - 714.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.