Annals of Botany 92: 289-297, 2003
© 2003 Annals of Botany Company
Diffusion Barriers of Tripartite Sporopollenin Microcapsules Prepared from Pine Pollen
1 Institut of Biology, Humboldt-University Berlin, Invalidenstr. 42, 10115 Berlin, Germany and 2 L.U.M. GmbH, Rudower Chaussee 29, 12489 Berlin, Germany
* For correspondence. E-mail rudolf.ehwald{at}rz.hu-berlin.de
Received: 7 February 2003; Returned for revision: 8 April 2003; Accepted: 7 May 2003
Tripartite sporopollenin microcapsules prepared from pine pollen (Pinus sylvestris L. and Pinus nigra Arnold) were analysed with respect to the permeability of the different strata of the exine which surround the gametophyte and form the sacci. The sexine at the surface of the sacci is highly permeable for polymer molecules and latex particles with a diameter of up to 200 nm, whereas the nexine covering the gametophyte is impermeable for dextran molecules, with a Stokes’ radius 
4 nm (Dextran T 70), and for the tetravalent anionic dye Evans Blue (Stokes’ radius = 1·3 nm). The central capsules obtained by dissolution of the sporoplasts showed strictly membrane-controlled exchange of non-electrolytes, with half-equilibration times in the range of minutes (monosaccharides, oligosaccharides) to hours (dextran molecules with Stokes’ radii up to 2·5 nm). The dependence of the permeability coefficients of the nexine for non-electrolytes on Stokes’ radius or molecular weight shows that the aqueous pores through the nexine are inhomogeneous with respect to their size, and that most pores are too narrow for free diffusion of sugar molecules. To explain the barrier function of the nexine for Evans Blue, it is assumed that at least the larger pores, which enable slow permeation of dextran molecules, contain negative charges.
Key words: Sporopollenin, exine, pores, size exclusion, permeability, sugars, dextran, Evans Blue, chromatography, pine, Pinus sylvestris L., Pinus nigra Arnold.
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