Annals of Botany 92: 557-563, 2003
© 2003 Annals of Botany Company
Root and Nodule Growth in Pisum sativum L. in Relation to Photosynthesis: Analysis Using 13C-labelling
1 INRA, Unité de Malherbologie et d’Agronomie, BV 86510, Dijon 21065 Cedex, France and 2 CEFE, CNRS, UPR 9056, 1919 route de Mende, 34293, Montpellier Cedex 5, France
* For correspondence. Fax 33 (0)3 80 69 32 62, e-mail salon{at}dijon.inra.fr
Received: 13 December 2002; Revised: 3 April 2003. Accepted: 23 June 2003
The effect of the nitrogen source (gaseous nitrogen, N2, or nitrate ions, NO3–) on the use of carbon (C) for root and nodule growth of pea (Pisum sativum L.) was investigated using 13C-labelling of assimilated CO2 at various stages of growth. Nitrate supply and growing conditions (sowing dates, air CO2 concentration) were varied to alter photosynthetic rates. Nodules are the sink with the highest demand for C in both the vegetative and flowering stages, growing at the expense of shoot and root in the vegetative stage, but only at the expense of roots at flowering. Until flowering, the addition of C into root and nodule biomass was linearly related to pre-existing biomass, thus determining net sink strengths which decreased with root and nodule age. Nodule growth patterns did not depend on the N source, whereas root growth was increased by nitrate when nodule biomass was low. At seed filling, the increase in C of biomass of the root system was no longer related to pre-existing biomass and C was preferentially diverted to roots of plants assimilating nitrate, or to nodules for plants fixing N2.
Key words: Roots, nodules, legume, growth, symbiotic N2 fixation, Pisum sativum L., 13C-labelling, photosynthesis.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  D. Moreau, A.-S. Voisin, C. Salon, and N. Munier-Jolain The model symbiotic association between Medicago truncatula cv. Jemalong and Rhizobium meliloti strain 2011 leads to N-stressed plants when symbiotic N2 fixation is the main N source for plant growth J. Exp. Bot., October 1, 2008; 59(13): 3509 - 3522. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A.-S. Voisin, V. Bourion, G. Duc, and C. Salon Using an Ecophysiological Analysis to Dissect Genetic Variability and to Propose an Ideotype for Nitrogen Nutrition in Pea Ann. Bot., December 1, 2007; 100(7): 1525 - 1536. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Bourion, G. Laguerre, G. Depret, A.-S. Voisin, C. Salon, and G. Duc Genetic Variability in Nodulation and Root Growth Affects Nitrogen Fixation and Accumulation in Pea Ann. Bot., September 1, 2007; 100(3): 589 - 598. [Abstract] [Full Text] [PDF]
|
|