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AOBPreview originally published online on October 8, 2003
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Annals of Botany 92: 749-755, 2003
© 2003 Annals of Botany Company

Association Between Chloroplast DNA and Mitochondrial DNA Haplotypes in Prunus spinosa L. (Rosaceae) Populations across Europe

APARAJITA MOHANTY{dagger},1, JUAN PEDRO MARTÍN*,1, LUIS MIGUEL GONZÁLEZ2 and ITZIAR AGUINAGALDE1

1 Departamento de Biología Vegetal, Escuela Técnica Superior de Ingenieros Agrónomos, Universidad Politécnica de Madrid, Ciudad Universitaria, 28040 Madrid, Spain and 2 Ministerio de Sanidad y Consumo, Instituto de Salud Carlos III, Centro Nacional de Microbiología, 28220 Majadahonda, Madrid, Spain

* For correspondence. E-mail jpmartin{at}bio.etsia.upm.es
{dagger} Present address: International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, 110067 New Delhi, India

Received: 4 April 2003; Returned for revision: 11 July 2003; Accepted: 9 August 2003    Published electronically: 8 October 2003

Chloroplast DNA (cpDNA) and mitochondrial DNA (mtDNA) were studied in 24 populations of Prunus spinosa sampled across Europe. The cpDNA and mtDNA fragments were amplified using universal primers and subsequently digested with restriction enzymes to obtain the polymorphisms. Combinations of all the polymorphisms resulted in 33 cpDNA haplotypes and two mtDNA haplotypes. Strict association between the cpDNA haplotypes and the mtDNA haplotypes was detected in most cases, indicating conjoint inheritance of the two genomes. The most frequent and abundant cpDNA haplotype (C20; frequency, 51 %) is always associated with the more frequent and abundant mtDNA haplotype (M1; frequency, 84 %). All but two of the cpDNA haplotypes associated with the less frequent mtDNA haplotype (M2) are private haplotypes. These private haplotypes are phylogenetically related but geographically unrelated. They form a separate cluster on the minimum-length spanning tree.

Key words: cpDNA haplotypes, mtDNA haplotypes, PCR–RFLP, phylogenetic relationship, Prunus spinosa L.


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