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Annals of Botany 93: 221-224, 2004
© 2004 Annals of Botany Company

Root Border Cells Take Up and Release Glucose-C

V. E. C. STUBBS1, D. STANDING*,2, O. G. G. KNOX2, K. KILLHAM2, A. G. BENGOUGH1 and B. GRIFFITHS1

1 Plant–Soil Interface Programme, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UKand 2 School of Biological Sciences, Cruickshank Building, University of Aberdeen, Aberdeen AB21 7AL, UK

* For correspondence. E-mail d.standing{at}abdn.ac.uk

Received: 6 August 2003;; Returned for revision: 3 October 2003; Accepted: 16 October 2003

ABSTRACT

• Background and Aims Border cells are released from the root tips of many plant species, and can remain viable in the rhizosphere for 1 week. Whether border cells are capable of controlled glucose exchange with their environment was investigated.

• Methods Border cells were removed from Zea mays L. root tips, and immersed in 14C-labelled D-glucose. In one experiment, the hexose transport inhibitor, phlorizin, was used to investigate active glucose uptake from a range of glucose concentrations. In another experiment, glucose efflux from border cells was monitored over time.

• Key Results Glucose uptake by the border cells increased with increasing glucose concentration from 0·2 to 20 mM. At 0·2 mM glucose, uptake was mainly active, as evidenced by the approx. 60 % inhibition with phlorizin. At 2 and 20 mM glucose, however, uptake was mainly via diffusion, as phlorizin inhibition was negligible. Glucose efflux increased with time for live border cells in both 2 and 20 mM glucose. There was no clear efflux/time pattern for heat-killed border cells.

• Conclusions Border cells actively take up glucose, and also release it. Under our experimental conditions, glucose uptake and efflux were of similar order of magnitude. In the rhizosphere net glucose exchange will almost certainly depend on local soil conditions.

Key words: glucose exchange, carbon flow, border cells, glucose uptake, sugar efflux.


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