AOBPreview originally published online on February 23, 2004
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Annals of Botany 93: 369-378, 2004
© 2004 Annals of Botany Company
Fast Pollen Tube Growth in Conospermum Species
1 Biological Sciences, Murdoch University, Murdoch, WA 6150, Australia, 2 Department of Agriculture Western Australia, South Perth, WA 6151, Australia and 3 Centre of Microscopy and Microanalysis, University of Western Australia, Nedlands WA 6907, Australia
* For correspondence. E-mail jmccomb{at}murdoch.edu.au
Received: 19 September 2003; Returned for revision: 6 November 2003; Accepted: 3 December 2003; Published electronically: 23 February 2004
• Background and Aims An unusual form of pollen tube growth was observed for several Conospermum species (family Proteaceae). The rate of pollen tube growth, the number of tubes to emerge and the ultrastructure of these tubes are given here.
• Methods Pollen was germinated in vitro in different sucrose concentrations and in the presence of calcium channel blockers, and tube emergence and growth were recorded on a VCR. Measurements were taken of the number of tubes to emerge and rate of tube emergence. Pollen behaviour in vivo was also observed. The ultrastructure of germinated and ungerminated pollen was observed using TEM.
• Key Results After 10 s to 3 min in germination medium, up to three pollen tubes emerged and grew at rates of up to 55 µm s–1; the rate then slowed to around 2 µm s–1, 30 s after the initial growth spurt. Tubes were observed to grow in pulses, and the pulsed growth continued in the presence of calcium channel blockers. Optimal sugar concentration for pollen germination was 300 g L–1, in which up to 81 % of pollen grains showed fast germination. Germination and emergence of multiple tubes were observed in sucrose concentrations of 100–800 g L–1. The vegetative and generative nuclei moved into one of the tubes. Multiple tubes from a single grain were observed on the stigma. Under light microscopy, the cytoplasm in the tube showed a clear region at the tip. The ultrastructure of C. amoenum pollen showed a bilayered exine, with the intine being very thick at the pores, and elsewhere having large intrusions into the plasma membrane. The cytoplasm was dense with vesicles packed with inner tube cell wall material. Golgi apparatus producing secretory vesicles, and mitochondria were found throughout the tube. The tube wall was bilayered; both layers being fibrous and loosely packed.
• Conclusions It is proposed that, for Conospermum, initial pollen tube wall constituents are manufactured and stored prior to pollen germination, and that tube extension occurs as described in the literature for other species, but at an exceptionally fast rate.
Key words: Pollen germination rate, multiple pollen tubes, pollen ultrastructure, calcium channel blockers, smokebush.
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