AOBPreview originally published online on May 14, 2004
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Annals of Botany 94: 87-97, 2004
© 2004 Annals of Botany Company
Phenylpropanoids, Phenylalanine Ammonia Lyase and Peroxidases in Elicitor-challenged Cassava (Manihot esculenta) Suspension Cells and Leaves
1 Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK
* For correspondence. E-mail: r.m.cooper{at}bath.ac.uk
2 Present address: Department of Agroecology in the Tropics and Subtropics, University of Honhenheim (308), D-70593, Germany
Received: 12 September 2003; Returned for revision: 5 November 2003; Accepted: 13 February 2003. Published electronically: 14 May 2004
• Background and aims Control of diseases in the key tropical staple, cassava, is dependent on resistant genotypes, but the innate mechanisms are unknown. The aim was to study phenylpropanoids and associated enzymes as possible defence components.
• Methods Phenylalanine ammonia-lyase (PAL), phenylpropanoids and peroxidases (POD) were investigated in elicited cassava suspension cells and leaves. Yeast elicitor was the most effective of several microbial and endogenous elicitors. Fungitoxicity was determined against the cassava pathogens Fusarium solani, F. oxysporum and the saprotroph Trichoderma harzianum.
• Key results A single and rapid (
2–3 min) oxidative burst, measured as hydrogen peroxide, occurred in elicited cells. PAL activity was induced maximally at 15 h and was preceded by PAL mRNA accumulation, which peaked at 9 h. Symplasmic POD activity increased four-fold in cells, 48 h post-elicitation. POD isoforms (2–7 isoforms, pI 3·1–8·8) were detected in elicited and unelicited cells, extracellular medium and leaves but two extracellular isoforms were enhanced post-elicitation. Also expression of a cassava peroxidase gene MecPOD1 increased in elicited cells. Only anionic forms oxidized scopoletin, with highest activity by isoform pI 3·6, present in all samples. Unidentified phenolics and possibly scopolin increased post-elicitation, but there was no enhancement of scopoletin, rutin or kaempferol-3-O-rutinoside concentration. Fungal germ tube elongation was inhibited more than germination by esculetin, ferulic acid, quercetin and scopoletin. T. harzianum was generally more sensitive than the pathogens and was inhibited by 50 µg mL–1 of ferulic acid and quercetin and 10 µg mL–1 of scopoletin.
• Conclusions Phenolic levels in cells were not enhanced and were, theoretically, too low to be inhibitory. However, in combination and when oxidized they may contribute to defence, because oxidation of esculetin and scopoletin by peroxidase and of esculetin by tyrosinase enhanced their fungitoxicity up to 20-fold.
Key words: Cassava, Manihot esculenta, phenylalanine ammonia-lyase, peroxidase, oxidative burst, phenylpropanoids, kaempherol-3-rutinoside, rutin, scopoletin, scopolin, yeast elicitor, plant defence.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. A. Farag, B. E. Deavours, A. de Fatima, M. Naoumkina, R. A. Dixon, and L. W. Sumner Integrated Metabolite and Transcript Profiling Identify a Biosynthetic Mechanism for Hispidol in Medicago truncatula Cell Cultures Plant Physiology, November 1, 2009; 151(3): 1096 - 1113. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Spitzer, M. M. B. Zvi, M. Ovadis, E. Marhevka, O. Barkai, O. Edelbaum, I. Marton, T. Masci, M. Alon, S. Morin, et al. Reverse Genetics of Floral Scent: Application of Tobacco Rattle Virus-Based Gene Silencing in Petunia Plant Physiology, December 1, 2007; 145(4): 1241 - 1250. [Abstract] [Full Text] [PDF]
|
|