Skip Navigation


AOBPreview originally published online on February 8, 2005
Annals of Botany 2005 95(5):789-797; doi:10.1093/aob/mci078
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
95/5/789    most recent
mci078v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (14)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by HENDRIX, B.
Right arrow Articles by STEWART, J. McD.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by HENDRIX, B.
Right arrow Articles by STEWART, J. McD.
Agricola
Right arrow Articles by HENDRIX, B.
Right arrow Articles by STEWART, J. McD.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?


© The Author 2005. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions{at}oupjournals.org

Estimation of the Nuclear DNA Content of Gossypium Species

BILL HENDRIX and JAMES McD. STEWART*

University of Arkansas, 115 Plant Science Building, Fayetteville, AR 72701, USA

* For correspondence. E-mail jstewart{at}uark.edu

Received: 9 August 2004    Returned for revision: 25 September 2004    Accepted: 30 November 2004    Published electronically: 8 February 2005

• Background and Aims Gossypium is an economically important, globally distributed taxon comprising more than 50 species. DNA content estimates from about half of the species indicate over a 3-fold variation exists. However, the nine DNA content estimates for G. hirsutum reveal over a 2-fold difference for this species alone. Recent reports have shown that several plant compounds can bias DNA content estimates obtained by commonly used methods. The purpose of this research was to examine the standardization procedures used for DNA content determinations with flow cytometry as applied to Gossypium, and generate revised DNA content estimates for all available Gossypium species using best-standard practices.

• Methods Flow cytometry was used to measure fluorescence of isolated Gossypium nuclei stained with propidium iodide. Fluorescence values were converted to DNA content estimates based on the nuclear fluorescence of standard genotypes of barley, corn and rice. Various combinations of nuclei preparations relative to the standards were evaluated for their influence on the estimates.

• Key Results Both external standardization and internal standardization with Oryza sativa ‘IR36’ yielded statistically similar DNA content estimates for Gossypium. Internal standardization with Hordeum vulgare ‘Sultan’ resulted in a high estimate of DNA content. Nuclear DNA content estimates were generated for 37 Gossypium species using external standardization. Estimates of ancestral genome sizes reveal that both increases and decreases in nuclear DNA content have occurred. Variation in intraspecific and intragenomic DNA content was low, and the allopolyploid AD-genome size was nearly the additive of its progenitor genomes.

• Conclusions Due to unknown factors, internal standardization with H. vulgare ‘Sultan’ may not be appropriate for DNA content determinations of Gossypium. The current DNA content estimates support accepted cytogenetic divisions of the genus. Gossypium is a genus that exhibits genome constancy both through speciation within genomic groups and allopolyploidization.

Key words: DNA content, Gossypium, flow cytometry, standardization, genome conservation, cytosolic interference, propidium iodide


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol Biol EvolHome page
C. E. Grover, Y. Yu, R. A. Wing, A. H. Paterson, and J. F. Wendel
A Phylogenetic Analysis of Indel Dynamics in the Cotton Genus
Mol. Biol. Evol., July 1, 2008; 25(7): 1415 - 1428.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
K. Wang, B. Guan, W. Guo, B. Zhou, Y. Hu, Y. Zhu, and T. Zhang
Completely Distinguishing Individual A-Genome Chromosomes and Their Karyotyping Analysis by Multiple Bacterial Artificial Chromosome-Fluorescence in Situ Hybridization
Genetics, February 1, 2008; 178(2): 1117 - 1122.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
J. J. Lee, A. W. Woodward, and Z. J. Chen
Gene Expression Changes and Early Events in Cotton Fibre Development
Ann. Bot., December 1, 2007; 100(7): 1391 - 1401.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Z. J. Chen, B. E. Scheffler, E. Dennis, B. A. Triplett, T. Zhang, W. Guo, X. Chen, D. M. Stelly, P. D. Rabinowicz, C. D. Town, et al.
Toward Sequencing Cotton (Gossypium) Genomes
Plant Physiology, December 1, 2007; 145(4): 1303 - 1310.
[Full Text] [PDF]

Home page
 Genome ResHome page
J. S. Hawkins, H. Kim, J. D. Nason, R. A. Wing, and J. F. Wendel
Differential lineage-specific amplification of transposable elements is responsible for genome size variation in Gossypium
Genome Res., October 1, 2006; 16(10): 1252 - 1261.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
J. LOUREIRO, E. RODRIGUEZ, J. DOLEZEL, and C. SANTOS
Flow Cytometric and Microscopic Analysis of the Effect of Tannic Acid on Plant Nuclei and Estimation of DNA Content
Ann. Bot., September 1, 2006; 98(3): 515 - 527.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Bot.Home page
E. Taliercio, B. Hendrix, and J. McD. Stewart
DNA content and expression of genes related to cell cycling in developing Gossypium hirsutum (Malvaceae) fibers
Am. J. Botany, December 1, 2005; 92(12): 1942 - 1947.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.