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AOBPreview originally published online on April 18, 2005
Annals of Botany 2005 96(1):91-99; doi:10.1093/aob/mci153
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© The Author 2005. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oupjournals.org

Widespread Occurrence of a Covalent Linkage Between Xyloglucan and Acidic Polysaccharides in Suspension-cultured Angiosperm Cells

ZOË A. POPPER and STEPHEN C. FRY*

The Edinburgh Cell Wall Group, Institute of Molecular Plant Sciences, School of Biological Sciences, The University of Edinburgh, Daniel Rutherford Building, The King's Buildings, Mayfield Road, Edinburgh EH9 3JH, UK

* For correspondence. E-mail S.Fry{at}ed.ac.uk

Received: 19 January 2005    Returned for revision: 17 February 2005    Accepted: 3 March 2005    Published electronically: 18 April 2005

• Background and Aims Covalent linkages between xyloglucan and rhamnogalacturonan-I (RG-I) have been reported in the primary cell walls of cultured Rosa cells and may contribute to wall architecture. This study investigated whether this chemical feature is general to angiosperms or whether Rosa is unusual.

• Methods Xyloglucan was alkali-extracted from the walls of L-[1-3H]arabinose-fed suspension-cultured cells of Arabidopsis, sycamore, rose, tomato, spinach, maize and barley. The polysaccharide was precipitated with 50 % ethanol and subjected to anion-exchange chromatography in 8 M urea. Eluted fractions were Driselase-digested, yielding [3H]isoprimeverose (diagnostic of [3H]xyloglucan). The Arabidopsis cells were also fed [6-14C]glucuronic acid, and radiolabelled pectins were extracted with ammonium oxalate.

• Key Results [3H]Xyloglucan was detected in acidic (galacturonate-containing) as well as non-anionic polysaccharide fractions. The proportion of the [3H]isoprimeverose units that were in anionic fractions was: Arabidopsis, 45 %; sycamore, 60 %; rose, 44 %; tomato, 75 %; spinach, 70 %; maize, 50 %; barley, 70 %. In Arabidopsis cultures fed D-[6-14C]glucuronate, 20 % of the (galacturonate-14C)-labelled pectins were found to hydrogen-bond to cellulose, a characteristic normally restricted to hemicelluloses such as xyloglucan.

• Conclusions Alkali-stable, anionic complexes of xyloglucan (reported in the case of Rosa to be xyloglucan–RG-I covalent complexes) are widespread in the cell walls of angiosperms, including gramineous monocots.

Key words: Dicots, gramineous monocots, pectin, cell wall (primary), xyloglucan–pectin linkage, hemicellulose, pectin, rhamnogalacturonan


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