AOBPreview originally published online on October 14, 2005
Annals of Botany 2005 96(7):1175-1189; doi:10.1093/aob/mci270
Morphological and Secretory Characterization of Extrafloral Nectaries in Plants of Coastal Veracruz, Mexico
1 Departamento de Ecología Funcional and 2 Departamento de Productos Forestales, Instituto de Ecología, A.C. Apdo. 63, Xalapa, Veracruz 91070, México
* For correspondence at Departamento de Ecología Tropical (CADET), Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Yucatán, Km 15.5 carretera Mérida-Xmatkuil, AP 4-116 Itzimná, Mérida, Yucatán 97100, México. E-mail diazcastelazo{at}yahoo.com.mx
Received: 8 October 2004 Returned for revision: 9 December 2004 Accepted: 10 August 2005 Published electronically: 14 October 2005
• Background and Aims Morphological descriptions of the extrafloral nectaries (EFNs) of certain plant species are common in the literature, but they rarely relate morphology with histology, gland distribution and secretory attributes. In this study a morphological/secretory characterization of EFNs occurring on several plant species in a tropical coastal community is made and the implications of gland attributes discussed from a functional perspective.
• Methods The morphology and nectar secretion of the EFNs of 20 plant species are characterized through scanning electron microscopy, histochemical detection of reducing sugars (Fehling's reagent) and nectar volume/concentration estimates.
• Key Results Sixty-five per cent of plant species in coastal communities had EFNs on vegetative structures and 35 % of species had glands on reproductive and vegetative organs. The Fabaceae is the plant family with the most species with EFNs and most diversity of gland morphologies. Four types of vascularized nectaries and four of glandular trichomes are described; sugar-secreting trichomes are characterized using Fehling's technique, and the first descriptions of unicellular and peltate trichomes functioning as EFNs are provided. Glands of ten plant species and six genera are described for the first time. Four plant species possess more than one morphological type of EFN. Eleven species have EFNs in more than one location or organ. More complex glands secrete more nectar, but are functionally homologous to the aggregations of numerous secretory trichomes on specific and valuable plant organs.
• Conclusion Important diversity of EFN morphology was foundin the coastal plant community studied. Both vascularized and non-vascularized EFNs are observed in plants and, for the latter, previously non-existent morpho-secretory characterizations are provided with a methodological approach to study them. It is recommended that studies relating EFN attributes (i.e. morphology, distribution) with their differential visitation by insects (i.e. ants) and the cost of maintenance to the plants are carried out to understand the evolution of these glands.
Key words: Extrafloral nectary morphology, secretory rates, nectary position, histology, taxonomy, ant-plant interactions, coastal plant communities