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AOBPreview originally published online on October 27, 2005
Annals of Botany 2006 97(1):29-37; doi:10.1093/aob/mcj005
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© The Author 2005. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Wound-induced Oxidative Responses in Mountain Birch Leaves

TEIJA RUUHOLA* and SHIYONG YANG

Section of Ecology, Department of Biology, University of Turku, FIN-20014 Turku, Finland

* For correspondence. E-mail teiruu{at}utu.fi

Received: 9 May 2005    Returned for revision: 28 July 2005    Accepted: 27 September 2005    Published electronically: 27 October 2005

• Aims The aim of the study was to examine oxidative responses in subarctic mountain birch, Betula pubescens subsp. czerepanovii, induced by herbivory and manual wounding.

• Methods Herbivory-induced changes in polyphenoloxidase, peroxidase and catalase activities in birch leaves were determined. A cytochemical dye, 3,3-diaminobenzidine, was used for the in situ and in vivo detection of H2O2 accumulation as a response to herbivory and wounding. To localize peroxidase activity in leaves, 10 mM H2O2 was applied to the dye reagent.

• Key Results Feeding by autumnal moth, Epirrita autumnata, larvae caused an induction in polyphenoloxidase and peroxidase activities within 24 h, and a concomitant decrease in the activity of antioxidative catalases in wounded leaves. Wounding also induced H2O2 accumulation, which may have both direct and indirect defensive properties against herbivores. Wound sites and guard cells showed a high level of peroxidase activity, which may efficiently restrict invasion by micro-organisms.

• Conclusion Birch oxidases together with their substrates may form an important front line in defence against herbivores and pathogens.

Key words: Defence, herbivory, peroxidase, polyphenoloxidase, mountain birch, Betula pubescens subsp. czerepanovii, autumnal moth, Epirrita autumnata


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