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AOBPreview originally published online on January 3, 2006
Annals of Botany 2006 97(5):903-915; doi:10.1093/aob/mcj604
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© The Author 2006. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Lateral Root Initiation in Arabidopsis: Developmental Window, Spatial Patterning, Density and Predictability

J. G. DUBROVSKY1,*, G. A. GAMBETTA1,2, A. HERNÁNDEZ-BARRERA1, S. SHISHKOVA1 and I. GONZÁLEZ1

1 Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apartado Postal 510-3, 62250 Cuernavaca, Morelos, Mexico and 2 Department of Viticulture and Enology, University of California, Davis, CA 95616, USA

* For correspondence. E-mail jdubrov{at}ibt.unam.mx

Received: 16 July 2005    Returned for revision: 21 September 2005    Accepted: 28 October 2005    Published electronically: 3 January 2006

• Background and Aims The basic regulatory mechanisms that control lateral root (LR) initiation are still poorly understood. An attempt is made to characterize the pattern and timing of LR initiation, to define a developmental window in which LR initiation takes place and to address the question of whether LR initiation is predictable.

• Methods The spatial patterning of LRs and LR primordia (LRPs) on cleared root preparations were characterized. New measures of LR and LRP densities (number of LRs and/or LRPs divided by the length of the root portions where they are present) were introduced and illustrate the shortcomings of the more customarily used measure through a comparative analysis of the mutant aux1-7. The enhancer trap line J0121 was used to monitor LR initiation in time-lapse experiments and a plasmolysis-based method was developed to determine the number of pericycle cells between successive LRPs.

• Key Results LRP initiation occurred strictly acropetally and no de novo initiation events were found between already developed LRs or LRPs. However, LRPs did not become LRs in a similar pattern. The longitudinal spacing of lateral organs was variable and the distance between lateral organs was proportional to the number of cells and the time between initiations of successive LRPs. There was a strong tendency towards alternation in LR initiation between the two pericycle cell files adjacent to the protoxylem poles. LR density increased with time due to the emergence of slowly developing LRPs and appears to be unique for individual Arabidopsis accessions.

• Conclusions. In Arabidopsis there is a narrow developmental window for LR initiation, and no specific cell-count or distance-measuring mechanisms have been found that determine the site of successive initiation events. Nevertheless, the branching density and lateral organ density (density of LRs and LRPs) are accession-specific, and based on the latter density the average distance between successive LRs can be predicted.

Key words: Root, lateral root, patterning, density, development, initiation


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