Skip Navigation


AOBPreview originally published online on July 24, 2006
Annals of Botany 2006 98(3):665-678; doi:10.1093/aob/mcl150
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Supplementary Information
Right arrow All Versions of this Article:
98/3/665    most recent
mcl150v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (9)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by SMARDA, P.
Right arrow Articles by BURES, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by SMARDA, P.
Right arrow Articles by BURES, P.
Agricola
Right arrow Articles by SMARDA, P.
Right arrow Articles by BURES, P.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?


© The Author 2006. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Intraspecific DNA Content Variability in Festuca pallens on Different Geographical Scales and Ploidy Levels

PETR SMARDA* and PETR BURES

Masaryk University, Faculty of Science, Institute of Botany and Zoology, Kotlárská 2, CZ-611 37 Brno, Czech Republic

* For correspondence. E-mail smardap{at}sci.muni.cz

Received: 25 April 2006    Returned for revision: 22 May 2006    Accepted: 26 May 2006    Published electronically: 24 July 2006

• Background and Aims Intraspecific genome size variability of Festuca pallens occurring on relict rocky steppes in Central Europe was studied on two ploidy levels and three geographical scales: (1) local scale of 24 populations, (2) landscape scale of three transects in river canyons or hill systems, and (3) global scale of 160 samples covering the whole distribution area.

• Methods DAPI flow cytometry of homogeneously cultivated samples (≥1 year), measured randomly with two internal standards, Lycopersicon esculentum and Pisum sativum. Differences in DNA content were confirmed (1) by the double peaks of simultaneously measured samples, (2) based on measurements carried out in different seasons, and (3) by additional measurements with propidium iodide.

• Key Results On a global scale, the relative DNA content ranged between 1·170-fold in diploids and 1·164-fold in tetraploids. A maximum difference of 1·088-fold between the mean relative DNA content of nearby populations was found. In 16 of 24 populations significant variability was shown (P < 0·001, 1·121-fold as maximum). For both ploidy levels, the relative genome size had the same range and geographical pattern, correlated with geographical coordinates (P < 0·01). Diploids with larger genomes occur on relict habitats (P < 0·01), and in areas of periglacial steppes (20 000 years ago; P < 0·02). In tetraploids, the relative DNA content differs among the three previously recognized geographical types (Alpine, Pannonian and Scabrifolia, P < 0·001). Tetraploids have a relative DNA content smaller than twice that of the diploids (P < 0·001). An influence of microhabitat on DNA content variation was not confirmed.

• Conclusions Genome size variability occurs over all spatial scales: intrapopulation, landscape and global. Correlation between geographical coordinates and palaeovegetation type, concomitant with diploids and tetraploids, and no influence of microhabitat were found. Genome size decreases in tetraploids. Lower CVs, and thus higher accuracy, resolution and reproducibility, favour DAPI measurements for the study of intraspecific genome size variability.

Key words: Festuca subgen. Festuca, fescue, Gramineae, polyploidy, Poaceae, genome size, flow cytometry, Central Europe, infraspecific C-value variation, DAPI, PI, DAPI/PI correlation


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 ANN BOT (LOND)Home page
P. Smarda, P. Bures, L. Horova, and O. Rotreklova
Intrapopulation genome size dynamics in Festuca pallens
Ann. Bot., August 5, 2008; (2008) mcn133v1.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
H. Saeidi, M. R. Rahiminejad, and J. S. Heslop-Harrison
Retroelement Insertional Polymorphisms, Diversity and Phylogeography within Diploid, D-genome Aegilops tauschii (Triticeae, Poaceae) Sub-taxa in Iran
Ann. Bot., April 1, 2008; 101(6): 855 - 861.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
P. Smarda, P. Bures, L. Horova, B. Foggi, and G. Rossi
Genome Size and GC Content Evolution of Festuca: Ancestral Expansion and Subsequent Reduction
Ann. Bot., February 1, 2008; 101(3): 421 - 433.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
J. Leong-Skornickova, O. Sida, V. Jarolimova, M. Sabu, T. Fer, P. Travnicek, and J. Suda
Chromosome Numbers and Genome Size Variation in Indian Species of Curcuma (Zingiberaceae)
Ann. Bot., September 1, 2007; 100(3): 505 - 526.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Bot.Home page
J. Suda, H. Weiss-Schneeweiss, A. Tribsch, G. M. Schneeweiss, P. Travnicek, and P. Schonswetter
Complex distribution patterns of di-, tetra-, and hexaploid cytotypes in the European high mountain plant Senecio carniolicus (Asteraceae)
Am. J. Botany, August 1, 2007; 94(8): 1391 - 1401.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
P. Smarda, P. Bures, and L. Horova
Random Distribution Pattern and Non-adaptivity of Genome Size in a Highly Variable Population of Festuca pallens
Ann. Bot., July 1, 2007; 100(1): 141 - 150.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.