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AOBPreview published online on May 7, 2009
Annals of Botany, doi:10.1093/aob/mcp101
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© The Author 2009. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

A phylogenetic study of Laeliinae (Orchidaceae) based on combined nuclear and plastid DNA sequences

Cássio van den Berg1,*, Wesley E. Higgins2, Robert L. Dressler3, W. Mark Whitten3, Miguel A. Soto-Arenas4 and Mark W. Chase5

1 Departamento de Ciências Biológicas, Universidade Estadual de Feira de Santana, Av. Transnordestina s/n, 44036-900, Feira de Santana, Bahia, Brazil
2 Marie Selby Botanical Gardens, 811 South Palm Avenue, Sarasota, FL 34236, USA
3 Florida Museum of Natural History, University of Florida, P.O. Box 110670, Gainesville, FL 32611-0670, USA
4 Instituto de Ecología, UNAM, Apartado Postal 70-275, México D.F. 04510, Mexico
5 Molecular Systematics Section, Jodrell Laboratory, Royal Botanic Gardens, Kew, Richmond, Surrey TW9 3DS, UK

* For correspondence. E-mail vcassio{at}gmx.de

Received: 4 August 2008    Returned for revision: 25 November 2008    Accepted: 24 March 2009   

Background and Aims: Laeliinae are a Neotropical orchid subtribe with approx. 1500 species in 50 genera. In this study, an attempt is made to assess generic alliances based on molecular phylogenetic analysis of DNA sequence data.

Methods: Six DNA datasets were gathered: plastid trnL intron, trnL-F spacer, matK gene and trnK introns upstream and dowstream from matK and nuclear ITS rDNA. Data were analysed with maximum parsimony (MP) and Bayesian analysis with mixed models (BA).

Key Results: Although relationships between Laeliinae and outgroups are well supported, within the subtribe sequence variation is low considering the broad taxonomic range covered. Localized incongruence between the ITS and plastid trees was found. A combined tree followed the ITS trees more closely, but the levels of support obtained with MP were low. The Bayesian analysis recovered more well-supported nodes. The trees from combined MP and BA allowed eight generic alliances to be recognized within Laeliinae, all of which show trends in morphological characters but lack unambiguous synapomorphies.

Conclusions: By using combined plastid and nuclear DNA data in conjunction with mixed-models Bayesian inference, it is possible to delimit smaller groups within Laeliinae and discuss general patterns of pollination and hybridization compatibility. Furthermore, these small groups can now be used for further detailed studies to explain morphological evolution and diversification patterns within the subtribe.

Key words: Laeliinae, Orchidaceae, ITS, trnL intron, trnL-F spacer, matK


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