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AOBPreview published online on October 8, 2009
Annals of Botany, doi:10.1093/aob/mcp254
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© The Author 2009. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Temporal regulation of cell-wall pectin methylesterase and peroxidase isoforms in cadmium-treated flax hypocotyl

Florence Paynel1,*, Annick Schaumann2, Mustapha Arkoun1, Olfa Douchiche1 and Claudine Morvan2

1 Laboratory Glyco-Mev, SCUEOR, IFRMP 23, University of Rouen, 76821 Mont Saint Aignan Cedex, France
2 Laboratory PBS-CNRS FRE3101, IFRMP 23, University of Rouen, 76821 Mont Saint Aignan Cedex, France

* For correspondence. E-mail florence.paynel{at}univ-rouen.fr

Received: 30 July 2009    Returned for revision: 27 August 2009    Accepted: 14 September 2009   

Background and Aims: In hypocotyls of flax (Linum usitatissimum) cadmium-induced reorientation of growth (i.e. an increase in expansion and a decrease in elongation) coincides with marked changes in the methylesterification and cross-linking of homogalacturonans within various cell-wall (CW) domains. The aim of the present study was to examine the involvement of pectin methylesterase (PME) and peroxidase (PER) in this cadmium-induced CW remodelling.

Methods: CW proteins were extracted from hypocotyls of 10- and 18-d-old flax that had been treated or not treated with 0·5 mM Cd(NO3)2. PME and PER expression within these extracts was detected by LC/MS, by isoelectric focusing and enzyme activity assays. Transcript expression by RT-PCR of known flax PME and PER genes was also measured in corresponding samples.

Key Results: In cadmium-treated seedlings, PME activity increased as compared with controls, particularly at day 10. The increased activity of PME was accompanied by increased abundance of both a basic protein isoform (B2) and a particular transcript (Lupme5). In contrast, induction of PER activity by cadmium was highest at day 18. Among the four reported PER genes, Flxper1 and 3 increased in abundance in the presence of cadmium at day 18.

Conclusions: The temporal regulation of Lupme and Flxper genes and of their respective enzyme activities fits the previously reported cadmium-induced structural changes of homogalacturonans within the CWs. After PME-catalysed de-esterification of homogalacturonans, their cross-linking would depend on the activity of PERs interacting with calcium-dimerized blocks and reinforce the cell cohesion during the cadmium-induced swelling.

Key words: Cadmium, cell wall, homogalacturonan, Linum usitatissimum, pectin methylesterase, peroxidase


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