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AOBPreview published online on December 1, 2009
Annals of Botany, doi:10.1093/aob/mcp283
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© The Author 2009. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Stereological estimation of cell wall density of DR12 tomato mutant using three-dimensional confocal imaging

David Legland1,*, Fabienne Guillon2, Kiên Kiêu3, Brigitte Bouchet2 and Marie-Françoise Devaux2

1 INRA UMR782 Food Process Engineering and Microbiology, F-78850 Thiverval-Grignon, France
2 INRA, UR1268 Biopolymers – Interactions and Assemblies, F-44300 Nantes, France
3 INRA, UR341 Applied Mathematics and Informatics, F-78350 Jouy-en-Josas, France

* For correspondence. E-mail david.legland{at}grignon.inra.fr

Received: 31 August 2009    Returned for revision: 13 October 2009    Accepted: 28 October 2009   

Background and Aims: The cellular structure of fleshy fruits is of interest to study fruit shape, size, mechanical behaviour or sensory texture. The cellular structure is usually not observed in the whole fruit but, instead, in a sample of limited size and volume. It is therefore difficult to extend measurements to the whole fruit and/or to a specific genotype, or to describe the cellular structure heterogeneity within the fruit.

Methods: An integrated method is presented to describe the cellular structure of the whole fruit from partial three-dimensional (3D) observations, involving the following steps: (1) fruit sampling, (2) 3D image acquisition and processing and (3) measurement and estimation of relevant 3D morphological parameters. This method was applied to characterize DR12 mutant and wild-type tomatoes (Solanum lycopersicum).

Key Results: The cellular structure was described using the total volume of the pericarp, the surface area of the cell walls and the ratio of cell-wall surface area to pericarp volume, referred to as the cell-wall surface density. The heterogeneity of cellular structure within the fruit was investigated by estimating variations in the cell-wall surface density with distance to the epidermis.

Conclusions: The DR12 mutant presents a greater pericarp volume and an increase of cell-wall surface density under the epidermis.

Key words: Stereology, 3D morphology estimation, tomato fruit, cellular structure, image analysis, confocal microscopy


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