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Variation in nuclear DNA content among coffee trees is an artefact

Noirot et al. (pp. 385-389) show that variation in nuclear DNA content within Coffea liberica var. dewevrei is an artefact due to cytoplasmic compounds limiting dye accessibility to DNA. This interference modifies the relationship between the fluorescence peak locations of the standard and the target. Interference differs among dyes, and stoichiometric errors may be minimized with propidium iodide, but not with DAPI.





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