
Environmental scanning microscopy reveals details of epidermal cell separation
Donald et al. (73-77) characterize in situ wall rupture and cell separation in hydrated Allium cepa tissue using the Environmental Scanning Electron Microscope. Cell rupture and release of contents are shown to take place at cellular junctions ahead of a propagating notch. However, cells that separate rather than rupture maintain their turgor. Strands of material between separating cells are compared with those between the layers of rupturing Chara corallina wall.