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AOBPreview originally published online on February 2, 2008
Annals of Botany 2008 101(5):689-699; doi:10.1093/aob/mcn008
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© The Author 2008. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

The Effects of Cold Acclimation on Photosynthetic Apparatus and the Expression of COR14b in Four Genotypes of Barley (Hordeum vulgare) Contrasting in their Tolerance to Freezing and High-light Treatment in Cold Conditions

Marcin Rapacz1,*, Barbara Wolanin1, Katarzyna Hura1 and MirosLaw Tyrka2

1 Agricultural University in Kraków, Faculty of Agriculture and Economics, Department of Plant Physiology, Podluzna 3, 30-239 Kraków, Poland
2 Polonia University, Faculty of Health Sciences, Laboratory for Population Genetics, Pulaskiego 4/6, 42-200 Czestochowa, Poland

* For correspondence. E-mail rrrapacz{at}cyf-kr.edu.pl

Received: 30 October 2007    Returned for revision: 4 December 2007    Accepted: 17 December 2007    Published electronically: 2 February 2008

Background and Aims: Cold acclimation modifies the balance of the energy absorbed and metabolized in the dark processes of photosynthesis, which may affect the expression of cold-regulated (COR) genes. At the same time, a gradual acclimation to the relatively high light conditions is observed, thereby minimizing the potential for photo-oxidative damage. As a result, the resistance to photoinhibition in the cold has often been identified as a trait closely related to freezing tolerance. Using four barley genotypes that differentially express both traits, the effect of cold acclimation on freezing tolerance and high-light tolerance was studied together with the expression of COR14b, one of the best-characterized barley COR genes.

Methods: Plants were cold acclimated for 2 weeks at 2 °C. Freezing tolerance was studied by means of electrolyte leakage. Changes in photosynthetic apparatus and high-light tolerance were monitored by means of chlorophyll fluorescence. Accumulation of COR14b and some proteins important in photosynthetic acclimation to cold were studied with western analysis. COR14b transcript accumulation during cold acclimation was assessed with real-time PCR.

Key Results: Cold acclimation increased both freezing tolerance and high-light tolerance, especially when plants were treated with high light after non-lethal freezing. In all plants, cold acclimation triggered the increase in photosynthetic capacity during high-light treatment. In two plants that were characterized by higher high-light tolerance but lower freezing tolerance, higher accumulation of COR14b transcript and protein was observed after 7 d and 14 d of cold acclimation, while a higher transient induction of COR14b expression was observed in freezing-tolerant plants during the first day of cold acclimation. High-light tolerant plants were also characterized with a higher level of PsbS accumulation and more efficient dissipation of excess light energy.

Conclusions: Accumulation of COR14b in barley seems to be important for resistance to combined freezing and high-light tolerance, but not for freezing tolerance per se.

Key words: Chlorophyll a fluorescence, cold acclimation, COR14b, non-photochemical energy quenching, freezing tolerance, gene expression, Hordeum vulgare, photoinhibition, photosynthetic acclimation to cold, photosynthetic capacity


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