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Annals of Botany 67: 517-519, 1991
© 1991 Annals of Botany Company

RESEARCH-ARTICLE

In Vitro Selection for Salt Tolerance in Brassica juncea L. Using Cotyledon Explants, Callus and Cell Suspension Cultures

R. K. JAIN, SUNITA JAIN* and J. B. CHOWDHURY

Department of Genetics, Haryana Agricultural University Hisar-125004, India
*Department of Chemistry and Biochemistry, Haryana Agricultural University Hisar-125004, India

Accepted: 21 January 1991   

Salt-tolerant Brassica juncea L. cell lines or plants have been selected by screening callus pieces, cell suspension cultures and cotyledon explants in vitro on high concentrations of NaCl. Callus-based selection was unsatisfactory, as only two out of seven isolated clones retained tolerance after 3 months of subculture on NaCl-free medium. Selections made via plated cell suspensions were found to be more stable for salt-tolerance. AH selected tolerant cell lines, however, failed to regenerate plantlets. A third selection method, employing cotyledon explants was based on their high potential for regenerating multiple shoots. Out of a total of 2620 explants cultured on high salt media, three survived, showed sustained callus proliferation and each regenerated one shoot. The salt-selected shoots withstood the stability test after 3 months of growth and axillary bud multiplication on NaCl-free medium. While one of these somaclones was morphologically abnormal and sterile, the other two could be reared to maturity with normal seed set.

Brassica juncea, tissue culture, in vitro selection, salt-tolerance, plant regeneration


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