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Annals of Botany 73: 515-523, 1994
© 1994 Annals of Botany Company

The Effect of Stratification and Thidiazuron Treatment on Germination and Protein Synthesis of Pyrus serotina Rehd cv. Niauli

Chin-Ho Lin, Ling-Yann Lee and Menq-Jiau Tseng

Department of Botany, and Department of Horticulture, National Chung Hsing University, Taichung, Taiwan, Republic of China

Breakage of seed dormancy in Pyrus serotina Rhed cv. Niauli is achieved by cold treatment. The chilling time necessary for germination of Pyrus serotina Rhed cv. Niauli seeds was determined, and synthesis of polypeptides during stratification and thidiazuron (TDZ) treatments was investigated. The chilling time required was about 21 d. The longer the chilling time, the higher the germination percentage. The effect of 200 µM TDZ on seed germination was equivalent to 14-21 d of chilling, and TDZ could be used as a substitute for cold treatment. Use of in vivo 35 S-methionine label and two-dimensional gel electrophoretic analysis of polypeptides revealed that the patterns of untreated (dormant) and chilled embryonic axes differed significantly in their polypeptide profiles. The difference became conspicuous after prolonged chilling exposure. During 28 d of stratification followed by 5 d of incubation at 25 °C, changes were noted in 35 soluble polypeptides (ESPs) and 38 pellet polypeptides (EPPs) from the embryonic axis. The changes included increase, decrease or newly induced synthesis. Common patterns of changes in soluble and pellet polypeptides of embryonic axes, can be broken down into four categories. The possible functions and roles of these four categories of polypeptides in breaking seed dormancy and germination are discussed. Although the magnitude of polypeptide changes are not as extensive in seeds receiving TDZ treatment as after stratification, similar polypeptide changes were co-induced by the two treatments.Copyright 1994, 1999 Academic Press

Stratification, protein synthesis, pear, Pyrus serotina, Thidiazuron, TDZ


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