Skip Navigation

This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (8)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Creemers-Molenaar, J.
Right arrow Articles by Gilissen, L. J.W.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Creemers-Molenaar, J.
Right arrow Articles by Gilissen, L. J.W.
Agricola
Right arrow Articles by Creemers-Molenaar, J.
Right arrow Articles by Gilissen, L. J.W.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Annals of Botany 73: 547-555, 1994
© 1994 Annals of Botany Company

Histology of the Morphogenic Response in Thin Cell Layer Explants from Vegetative Tobacco Plants

J. Creemers-Molenaar, J. C. Hakkert, M. J. Van Staveren and L. J.W. Gilissen

Department of Cell Biology, DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA Wageningen, The Netherlands

The morphogenic response of thin cell layers (TCLs) from vegetative tobacco (Nicotiana tabacum L.) plants can be directed very precisely by varying the concentrations of benzyladenine (BA) and {alpha}-naphthalene acetic acid (NAA) in the culture medium. Medium containing 1·6 µM BA and 0·5 µM NAA was optimal for shoot formation, concentrations of 0·5 µM BA and 1·6 µM NAA were optimal for the induction of shoots and roots on the same explant, whereas concentrations of NAA higher than 16 µM resulted in callus proliferation only. Polarity in the distribution of the shoot buds was observed, i.e. a switch from basal to apical shoot formation occurred with increasing NAA concentrations, suggesting basipetal transport of NAA. Histological examination of TCLs on shoot induction medium revealed that first cell divisions occurred within 2 d in cortical cells which were directly in contact with the medium along the longitudinal cut surface, and after 2 d in subepidermal cells along the lateral edges of the explants. Individual lateral buds originated from one subepidermal and one or more epidermal cells, while apical buds originated from single subepidermal or cortical cells located directly at the apical end of the explant. After culture of TCLs for 2-3 d on root/shoot induction medium cells in the regeneration-competent subepidermis elongated, while on callus induction medium subepidermal cells elongated and dedifferentiated. The regeneration system as described in this study will be used to identify cells competent for regeneration as well as for transformation.Copyright 1994, 1999 Academic Press

Nicotiana tabacum L., tobacco, thin cell layer explants, cell competence, shoot development, polarity


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.