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Annals of Botany 75: 477-480, 1995
© 1995 Annals of Botany Company

In vitro Hormonal Regulation of Laticifer Differentiation in Calotropis procera

S. S. Suri and K. G. Ramawat

Laboratory of Bio-Molecular Technology, Department of Botany, M. L. Sukhadia University, Udaipur-313 001, India

Cultures of Calotropis procera were maintained on MS medium supplemented with 4·6 µM FAP and 3 µM NAA. Laticifer initials were observed in 2-week-old cultures which were converted into matured, branched, non-articulated laticifers in 4 weeks. It was observed that laticifer differentiation increased with the age of cultures, from 2·78% (in the second passage) to 15·11% (in the 12th passage). It has been established that laticifer differentiation in vitro is a cytokinin-dependent process and among the cytokinins, FAP was more effective than BA and 2-iP. But the type of auxin and its concentration also play an important role in modifying the effect of cytokinin. Among the different auxins used IAA was more effective for laticifer differentiation than IBA and NAA, while 2,4D was inhibitory. Maximum laticifer differentiation (17·01% was observed on MS medium supplemented with 4·6 µM FAP and 1 µM IAA.Copyright 1995, 1999 Academic Press

Calotropis procera, callus culture, laticifer, differentiation, hormonal regulation


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