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Annals of Botany 75: 579-585, 1995
© 1995 Annals of Botany Company

Effect of Different Seed Storage Conditions on Germination and Isozyme Activity in Some Brassica Species

M. C. Ramiro, F. Pérez-García and I. Aguinagalde

Departamento de Biología Vegetal, Escuela Técnica Superior de Ingenieros Agrónomos, Universidad Politécnica de Madrid, Ciudad Universitaria, 28040 Madrid, Spain

Sixteen seed accessions of four Brassica species (B. cretica, B. incana, B. montana and B. oleracea) were examined following 5-22 years' storage in the germplasm bank. Germinability and isozyme of seeds stored under long-term (-10 °C and 3% moisture content) and short-term (5% °C and 8% moisture content) storage conditions were compared. Long-term storage produced no deterioration and the ability to germinate was satisfactorily maintained over 8-22 years. Short-term storage conditions maintained germination ability up to 10-12 years in all accessions of B. cretica and B. montana. However, seed multiplication might be essential every 10 years for some accessions of B. oleracea stored in this way. In the 8 to 9-year-old accessions of B. cretica, B. incana and B. montana, no significant differences were detected between conservation systems for germinability and frequency of seeds showing isozyme activity for seven enzyme systems (ACO, IDH, MDH, ME, PGI, PGM and 6-PGD). However, significant differences were found for the ADH enzyme system. Moreover, in the 5-22-year-old B. oleracea accessions, significant differences were found between storage conditions with respect to isozyme activity for all enzyme systems studied.Copyright 1995, 1999 Academic Press

Brassica cretica, Brassica incana, Brassica montana, Brassica oleracea, accessions, isozymes, germination, seed storage


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