Skip Navigation

This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (21)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by FAURE, O.
Right arrow Articles by NOUGARÈDE, A.
Right arrow Search for Related Content
PubMed
Right arrow Articles by FAURE, O.
Right arrow Articles by NOUGARÈDE, A.
Agricola
Right arrow Articles by FAURE, O.
Right arrow Articles by NOUGARÈDE, A.
Social Bookmarking
 Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Annals of Botany 78: 23-28, 1996
© 1996 Annals of Botany Company

Ontogenesis, Differentiation and Precocious Germination in Anther-derived Somatic Embryos of Grapevine (Vitis vinifera L.): Proembryogenesis

O. FAURE+, J. AARROUF and A. NOUGARÈDE

Laboratoire de Biologie Végétale, Université Jean Monnet, 23 rue du Dr Michelon, F-42023 St-Etienne Cedex 2, France Laboratoire de Cytologie Expérimentale et Morphogenèse Végétale, Université P. et M. Curie, Bat. N2, 4 place Jussieu, F-75252, Paris Cedex 05, France

April 11, 1995 ; December 23, 1995

Histological steps of callogenesis and proembryogenesis in anther cultures ofVitis vinifera L. ‘Grenache noir’ are described. Embryogenic calli were obtained on Nitsch and Nitsch medium supplemented with 1mgl-12,4-dichlorophenoxyacetic acid (2,4-D) and 0.25mgl-1benzylaminopurine (BAP). Calli were initiated from anther connective cells only and no division of microspores occurred. The embryos were hence of somatic origin. Proembryos developed either directly (i.e. without intervening callus) from the endothecium, or indirectly from the connective-derived callus. In both cases, proembryos originated from single cells. They developed from starchy differentiated cells of a predetermined type. The polarity of the somatic proembryo was established from the first divisions and it was marked by precocious development of an easily recognizable suspensor. Other analogies with the development of the zygote are also emphasised.

Vitis vinifera L.; grapevine; somatic embryogenesis; proembryogenesis; histology


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
Am. J. Bot.Home page
S. Jayasankar, B. R. Bondada, Z. Li, and D. J. Gray
Comparative anatomy and morphology of Vitis vinifera (Vitaceae) somatic embryos from solid- and liquid-culture-derived proembryogenic masses
Am. J. Botany, July 1, 2003; 90(7): 973 - 979.
[Abstract] [Full Text] [PDF]



Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.