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Annals of Botany 81: 109-114, 1998
© 1998 Annals of Botany Company

Biolistic Transformation of Rose (Rosa hybridaL.)

R. MARCHANT+,, J. B. POWER, J. A. LUCAS and M. R. DAVEY

Plant Genetic Manipulation Group, Department of Life Science, University of Nottingham, University Park, Nottingham, NG7 2RD, UK IACR-Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, Long Ashton, Bristol, BS19 9AF, UK

June 2, 1997 ; September 17, 1997 .

A reproducible method has been developed for the Biolistic transformation and regeneration of transgenic plants from embryogenic callus of rose (Rosa hybridaL.) cv. Glad Tidings. DNA delivery was optimized using the ß-glucuronidase (gus) gene. The distance between the stopping screen and target explants and supplementation of pre-and post-bombardment culture media with 0.25Mmyo-inositol influenced the transformation efficiency. Prior to culture on selection medium containing 250 mg l-1kanamycin sulphate, embryogenic calli were bombarded, using optimized gene delivery parameters, with a plasmid carrying the neomycin phosphotransferase (nptII) gene. Somatic embryo-derived kanamycin-resistant plants were regenerated and subsequently transferred to glasshouse conditions. Transformation was confirmed by kanamycin resistance of calli and plants, NPT II ELISA assay and Southern analysis. All transgenic plants were morphologically normal (true-to-type).Copyright 1998 Annals of Botany Company

Biolistic; genetic engineering; rose;Rosa hybridaL.; transformation.


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