Skip Navigation

This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (2)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by GROTE, M.
Right arrow Articles by WITZEL, H.
Right arrow Search for Related Content
PubMed
Right arrow Articles by GROTE, M.
Right arrow Articles by WITZEL, H.
Agricola
Right arrow Articles by GROTE, M.
Right arrow Articles by WITZEL, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Annals of Botany 82: 235-241, 1998
© 1998 Annals of Botany Company

Localization of the Fe(III)-Zn(II) Purple Acid Phosphatase in Dry Kidney Beans Using Immunofluorescence and Immunogold Electron Microscopy

M. GROTE, C. SCHWERDTFEGER, R. WIERMANN+,, R. REICHELT and H. WITZEL

Institut für Medizinische Physik und Biophysik, Westfälische Wilhelms-Universität, Münster, Germany Institut für Botanik, Westfälische Wilhelms-Universität, Münster, Germany Institut für Biochemie, Westfälische Wilhelms-Universität, Münster, Germany

March 10, 1998 ; March 26, 1998 . April 30, 1998 .

Localization of purple acid phosphatase (PAP) from the seeds of kidney beans,Phaseolus vulgaris(L.), was performed using light and transmission electron microscopy. After rehydration and aqueous fixation, cryo-sections of bean cotyledon tissue showed a bright immunofluorescent signal in the cytoplasm of cells whereas cell walls and reserve materials (starch, protein bodies) remained unstained. In ultrathin sections of dry cotyledon tissue anhydrously fixed in acrolein vapour and embedded in Lowicryl resin, PAP mapped exclusively to ribosome-rich areas of the cytoplasm. In view of these results, we propose that kidney bean PAP might possibly be engaged in mechanisms involved in the triggering of seed dormancy.Copyright 1998 Annals of Botany Company

Phaseolus vulgaris(L.), kidney bean, purple acid phosphatase, immunofluorescence microscopy, immunogold electron microscopy, anhydrous vapour fixation, acrolein.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.