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Annals of Botany 89: 77-82, 2002
© 2002 Annals of Botany Company

Expression Pattern of Aux/IAA Genes in the iaa3/shy2-1D Mutant of Arabidopsis thaliana (L.)

YUTAKA OONO*,1, CHIHARU OOURA1 and HIROFUMI UCHIMIYA1,2

1Advanced Science Research Center, Japan Atomic Energy Research Institute (JAERI), Takasaki, Gunma 370-1292, Japan and 2Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo-ku, Tokyo 113-0032, Japan

 * For correspondence. Fax +81-273-346-9696, e-mail yoono{at}taka.jaeri.go.jp

Received: 18 June 2001; Returned for revision: 1 August 2001; Accepted: 17 September 2001.

A semi-dominant mutant suppressor of hy2 (shy2-1D) of Arabidopsis thaliana, originally isolated as a photomorphogenesis mutant, shows altered auxin responses. Recent molecular cloning revealed that the SHY2 gene is identical to the IAA3 gene, a member of the primary auxin-response genes designated the Aux/IAA gene family. Because Aux/IAA proteins are reported to interact with auxin response factors, we investigated the pattern of expression of early auxin genes in the iaa3/shy2-1D mutant. RNA hybridization analysis showed that levels of mRNA accumulation of the early genes were reduced dramatically in the iaa3/shy2-1D mutants, although auxin still enhanced gene expression in the iaa3/shy2-1D mutant. Histochemical analysis using a fusion gene of the auxin responsive domain (AuxRD) and the GUS gene showed no IAA-inducible GUS expression in the root elongation zone of the iaa3/shy2-1D mutant. On the other hand, ectopic GUS expression occurred in the hypocotyl, cotyledon, petiole and root vascular tissues in the absence of auxin. These results suggest that IAA3/SHY2 functions both negatively and positively on early auxin gene expression.

Key words: Arabidopsis thaliana, auxin, Aux/IAA genes, gene expression, GUS expression, IAA, IAA3, indole-3-acetic acid, mutants, shy2-1.


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