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Annals of Botany 89: 627-636, 2002
© 2002 Annals of Botany Company

Characterization of a Genetic Resource Collection for Miscanthus (Saccharinae, Andropogoneae, Poaceae) using AFLP and ISSR PCR

TREVOR R. HODKINSON*,1,2, MARK W. CHASE1 and STEPHEN A. RENVOIZE1

1Royal Botanic Gardens, Kew, Richmond, Surrey TW9 3AB, UK and 2Department of Botany, Trinity College, Dublin 2, Ireland

* For correspondence. Fax +353 1 6081147, e-mail trevor.hodkinson{at}tcd.ie

Received: 27 October 2000; Returned for revision: 13 April 2001; Accepted: 4 February 2002.

Amplified fragment length polymorphism (AFLP) and inter-simple sequence repeat markers were employed to characterize a genetic resource collection of Miscanthus, a grass under trial in Europe as a biomass crop. The 26 polymorphic markers produced by two ISSR fingerprinting primers were able to discriminate taxa and identify putative clones. AFLP fingerprints were fully reproducible and produced a larger number of markers for the three primer pairs tested, of which 998 were polymorphic (representing 79·3 % of all bands). AFLP markers distinguished species, infra-specific taxa (varieties and cultivars) and putatively clonal material. They were also used to assess the inter-relationships of the taxa, to investigate the origin of important hybrid plants and to estimate the overall level of genetic variation in the collection. They were useful for assessing the species status of certain taxa such as M. transmorrisonensis, an endemic from Taiwan that was clearly distinct from M. sinensis; whereas other taxa of disputed species status, such as M. condensatus and M. yakushimanum were not genetically distinct from M. sinensis. The AFLP markers detected a high degree of infra-specific variation and allowed subdivisions of the genetic resource collection to be made, particularly within M. sinensis.

Key words: Miscanthus, AFLP, ISSR, inter-microsatellites, biomass, genetic resources, taxonomy, hybrids, molecular phylogenetics.


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