Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Content Snapshot
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (6)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by ANDERSONE, U.
Right arrow Articles by IEVINSH, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by ANDERSONE, U.
Right arrow Articles by IEVINSH, G.
Agricola
Right arrow Articles by ANDERSONE, U.
Right arrow Articles by IEVINSH, G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Annals of Botany 90: 293-298, 2002
© 2002 Annals of Botany Company

Changes of Morphogenic Competence in Mature Pinus sylvestris L. Buds in vitro

UNA ANDERSONE1 and GEDERTS IEVINSH*,1

1 Department of Plant Physiology, Institute of Biology, University of Latvia, 3 Miera Str., LV-2169 Salaspils, Latvia

* For correspondence. Fax +371 7944986, e-mail gederts{at}e-apollo.lv

Received: 7 January 2002; Returned for revision: 26 April 2002; Accepted: 9 May 2002

ABSTRACT

The effects of season and cold storage on morphogenic competence in mature Pinus sylvestris buds were investigated. Peroxidase and polyphenol oxidase activity were measured as markers of oxidative metabolism. No growth in vitro was observed on explants detached from the end of January until the beginning of March. Brachioblasts, each with a couple of needles, formed on 11 % of the buds without macrostrobili that were detached in early April and introduced immediately into culture. Of the explants detached in late July, 15 % formed shoots with brachioblasts and needles. The lowest activity of peroxidase and polyphenol oxidase in pine buds was observed from the end of April until the beginning of June when morphogenic competence of tissues started to increase. Development of bud explants detached in January was achieved by cold storage for 5 months. Low polyphenol oxidase and peroxidase activity coincided with increased morphogenic potential. Results suggest that reduced or stable activity of peroxidase and polyphenol oxidase is associated with an increased ability of tissues to start growth in vitro.

Key words: Pinus sylvestris L., Scots pine, buds, morphogenic competence, peroxidase, polyphenol oxidase, seasonal changes, cold storage.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.