AOBPreview originally published online on August 5, 2002
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Annals of Botany 90: 345-351, 2002
© 2002 Annals of Botany Company
Intra- and Interspecific Variation in DNA Content in Cistus (Cistaceae)
1 Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia, Camino de Vera s/n. 46022 Valencia, Spain and 2 Jardín Botánico, Universidad de Valencia, Calle Quart 80, 46008 Valencia, Spain
* For correspondence. Fax +34 96 3156826, e-mail Monica.Boscaiu{at}uv.es
Received: 13 November 2002; Returned for revision: 6 March 2002; Accepted: 30 May 2002
Flow cytometry, using propidium iodide and 4',6-diamidano-2-phenylindole staining, was used to estimate the nuclear DNA content (2C) and the proportion of A–T base pairs in 16 species of the Mediterranean genus Cistus. Genome sizes were shown to be constant within species, since no significant intraspecific variation in 2C DNA content was detected. At the genus level, up to about 1·5-fold differences in absolute DNA amounts were observed, ranging from 3·92 pg in C. crispus to 5·88 pg in C. monspeliensis. The (AT) : (GC) ratio was close to 1, and was similar for all species examined, ranging from 47·87 % A–T content in C. clusii, to 50·67 % in C. populifolius. Pink-flowered species (subgenus Cistus) had lower DNA amounts than white-flowered species (subgenera Leucocistus and Halimioides). However, the distribution of DNA amounts in Cistus appeared to be continuous and did not permit a clear separation of infra-generic ranks in the genus.
Key words: Flow cytometry, nuclear DNA content, genome size, A–T content, Cistus, Cistaceae.
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