Skip Navigation


AOBPreview originally published online on November 13, 2002
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Content Snapshot
Right arrow All Versions of this Article:
91/1/49    most recent
mcg007v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (7)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by SONNANTE, G.
Right arrow Articles by PIGNONE, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by SONNANTE, G.
Right arrow Articles by PIGNONE, D.
Agricola
Right arrow Articles by SONNANTE, G.
Right arrow Articles by PIGNONE, D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Annals of Botany 91: 49-54, 2003
© 2003 Annals of Botany Company

ITS Sequence Analysis and Phylogenetic Inference in the Genus Lens Mill.

GABRIELLA SONNANTE*,1, INCORONATA GALASSO1 and DOMENICO PIGNONE1

1 C.N.R. Istituto del Germoplasma, Via Amendola 165/A, 70126 Bari, Italy

* For correspondence. Fax +39 080 558 75666, e-mail germgs15{at}area.ba.cnr.it

Received: 21 March 2002; Returned for revision: 11 August 2002; Accepted: 2 October 2002    Published electronically: 13 November 2002

The internal transcribed spacer (ITS) region of the nuclear ribosomal DNA from cultivated lentil (Lens culinaris subsp. culinaris) and its wild relatives was isolated and analysed for nucleotide sequence variation. Sequence divergence values ranged from no polymorphism within single species and between the cultigen and one accession of its wild progenitor (L. culinaris subsp. orientalis) to 14 base substitutions between L. nigricans and L. lamottei. Jukes and Cantor distance ranged from 0 to 1·79 %. Phylogenetic analysis confirmed the divergence of L. nigricans from all species, and the closeness of cultivated lentil to its wild progenitor, although two gene pools could possibly be identified in subsp. orientalis. Based on this study, the two recently recognized species, L. lamottei and L. tomentosus were separated from the other species. Each wild species showed peculiar autapomorphies and, in general, did not display much variation among accessions. The trees using chickpea as an outgroup formed two main clusters, one constituted by L. nigricans only and the other including the remaining taxa. Within this larger group, small subclades could be identified.

Key words: Lens culinaris Medik. subsp. culinaris, lentil, wild species, ITS sequence, polymorphism, parsimony, phylogenetic relationships.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.