Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Content Snapshot
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (16)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by MOSCONE, E. A.
Right arrow Articles by HUNZIKER, A. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by MOSCONE, E. A.
Right arrow Articles by HUNZIKER, A. T.
Agricola
Right arrow Articles by MOSCONE, E. A.
Right arrow Articles by HUNZIKER, A. T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Annals of Botany 92: 21-29, 2003
© 2003 Annals of Botany Company

Analysis of Nuclear DNA Content in Capsicum (Solanaceae) by Flow Cytometry and Feulgen Densitometry

EDUARDO A. MOSCONE*,1, MONIKA BARANYI2, IRMA EBERT{dagger},2, JOHANN GREILHUBER2, FRIEDRICH EHRENDORFER2 and ARMANDO T. HUNZIKER{dagger},1

1 Instituto Multidisciplinario de Biología Vegetal (IMBIV), Casilla de Correo 495, 5000 Córdoba, Argentina and 2 Institute of Botany and Botanical Garden of the University of Vienna, Rennweg 14, A-1030 Vienna, Austria

* For correspondence. Fax 0054 351 4332104, e-mail moscone{at}imbiv.unc.edu.ar
{dagger} Deceased.

Received: 11 September 2002; Returned for revision: 6 January 2003; Accepted: 4 March 2003

Flow cytometric measurements of nuclear DNA content were performed using ethidium bromide as the DNA stain (internal standard, Hordeum vulgare ‘Ditta’, 1C = 5·063 pg) in 25 samples belonging to nine diploid species and four varieties of Capsicum: C. chacoense, C. parvifolium, C. frutescens, C. chinense, C. annuum var. annuum, C. baccatum var. baccatum, C. baccatum var. pendulum, C. baccatum var. umbilicatum, C. eximium and C. pubescens, all with 2n = 24, and C. campylopodium with 2n = 26. In addition, one sample each of C. annuum var. annuum and C. pubescens were also analysed using Feulgen densitometry (standard, Allium cepa ‘Stuttgarter Riesen’, 1C = 16·75 pg). Both staining methods resulted in very similar relative values. Genome size displays significant variation between but not within species (except in C. campylopodium), and contributes to their taxonomic grouping. 1C-values range from 3·34–3·43 pg (3273–3361 Mbp) in C. chacoense and the C. annuum complex to 4·53–5·77 pg (4439–5655 Mbp) in C. campylopodium and C. parvifolium. The data obtained support conclusions on phylogenetic relationships in the genus derived from karyotype analyses using chromosome banding approaches. In Capsicum, constitutive heterochromatin amount is correlated with genome size, except in C. parvifolium, and is regarded as an additive genomic component.

Key words: Solanaceae, Capsicum, genome size variation, flow cytometry, Feulgen densitometry, karyosystematics, karyotype evolution.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 ANN BOT (LOND)Home page
J. Chrtek Jr, J. Zahradnicek, K. Krak, and J. Fehrer
Genome size in Hieracium subgenus Hieracium (Asteraceae) is strongly correlated with major phylogenetic groups
Ann. Bot., July 1, 2009; 104(1): 161 - 178.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
J. DOLEZEL and J. BARTOS
Plant DNA Flow Cytometry and Estimation of Nuclear Genome Size
Ann. Bot., January 1, 2005; 95(1): 99 - 110.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
B. G. MURRAY
When does Intraspecific C-value Variation become Taxonomically Significant?
Ann. Bot., January 1, 2005; 95(1): 119 - 125.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.