Annals of Botany 95/1 © Annals of Botany Company 2005; all rights reserved
Nuclear DNA Amounts in Angiosperms: Progress, Problems and Prospects
Jodrell Laboratory, Royal Botanic Gardens, Kew, Richmond, Surrey TW9 3AB, UK
* For correspondence. E-mail m.bennett{at}kew.org
Received: 23 July 2004 Returned for revision: 12 August 2004 Accepted: 1 September 2004
|
• Background The nuclear DNA amount in an unreplicated haploid chromosome complement (1C-value) is a key diversity character with many uses. Angiosperm C-values have been listed for reference purposes since 1976, and pooled in an electronic database since 1997 (http://www.kew.org/cval/homepage). Such lists are cited frequently and provide data for many comparative studies. The last compilation was published in 2000, so a further supplementary list is timely to monitor progress against targets set at the first plant genome size workshop in 1997 and to facilitate new goal setting.
• Scope The present work lists DNA C-values for 804 species including first values for 628 species from 88 original sources, not included in any previous compilation, plus additional values for 176 species included in a previous compilation.
• Conclusions 1998–2002 saw striking progress in our knowledge of angiosperm C-values. At least 1700 first values for species were measured (the most in any five-year period) and familial representation rose from 30 % to 50 %. The loss of many densitometers used to measure DNA C-values proved less serious than feared, owing to the development of relatively inexpensive flow cytometers and computer-based image analysis systems. New uses of the term genome (e.g. in ‘complete’ genome sequencing) can cause confusion. The Arabidopsis Genome Initiative C-value for Arabidopsis thaliana (125 Mb) was a gross underestimate, and an exact C-value based on genome sequencing alone is unlikely to be obtained soon for any angiosperm. Lack of this expected benchmark poses a quandary as to what to use as the basal calibration standard for angiosperms. The next decade offers exciting prospects for angiosperm genome size research. The database (http://www.kew.org/cval/homepage) should become sufficiently representative of the global flora to answer most questions without needing new estimations. DNA amount variation will remain a key interest as an integrated strand of holistic genomics.
Key words: Angiosperm DNA amounts, DNA C-values, nuclear genome size, plant DNA C-values database
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Salse, M. Abrouk, S. Bolot, N. Guilhot, E. Courcelle, T. Faraut, R. Waugh, T. J. Close, J. Messing, and C. Feuillet Reconstruction of monocotelydoneous proto-chromosomes reveals faster evolution in plants than in animals PNAS, September 1, 2009; 106(35): 14908 - 14913. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Whittle and J. E. Krochko Transcript Profiling Provides Evidence of Functional Divergence and Expression Networks among Ribosomal Protein Gene Paralogs in Brassica napus PLANT CELL, August 1, 2009; 21(8): 2203 - 2219. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. E. Grover, Y. Yu, R. A. Wing, A. H. Paterson, and J. F. Wendel A Phylogenetic Analysis of Indel Dynamics in the Cotton Genus Mol. Biol. Evol., July 1, 2008; 25(7): 1415 - 1428. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. D. Morin, G. Aksay, E. Dolgosheina, H. A. Ebhardt, V. Magrini, E. R. Mardis, S. C. Sahinalp, and P. J. Unrau Comparative analysis of the small RNA transcriptomes of Pinus contorta and Oryza sativa Genome Res., April 1, 2008; 18(4): 571 - 584. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. D. Bennett, H. J. Price, and J. S. Johnston Anthocyanin Inhibits Propidium Iodide DNA Fluorescence in Euphorbia pulcherrima: Implications for Genome Size Variation and Flow Cytometry Ann. Bot., April 1, 2008; 101(6): 777 - 790. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Saeidi, M. R. Rahiminejad, and J. S. Heslop-Harrison Retroelement Insertional Polymorphisms, Diversity and Phylogeography within Diploid, D-genome Aegilops tauschii (Triticeae, Poaceae) Sub-taxa in Iran Ann. Bot., April 1, 2008; 101(6): 855 - 861. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Leong-Skornickova, O. Sida, V. Jarolimova, M. Sabu, T. Fer, P. Travnicek, and J. Suda Chromosome Numbers and Genome Size Variation in Indian Species of Curcuma (Zingiberaceae) Ann. Bot., September 1, 2007; 100(3): 505 - 526. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. F. Kapraun Nuclear DNA Content Estimates in Green Algal Lineages: Chlorophyta and Streptophyta Ann. Bot., April 1, 2007; 99(4): 677 - 701. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Neumann, A. Koblizkova, A. Navratilova, and J. Macas Significant Expansion of Vicia pannonica Genome Size Mediated by Amplification of a Single Type of Giant Retroelement Genetics, June 1, 2006; 173(2): 1047 - 1056. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Hasterok, A. Marasek, I. S. Donnison, I. Armstead, A. Thomas, I. P. King, E. Wolny, D. Idziak, J. Draper, and G. Jenkins Alignment of the Genomes of Brachypodium distachyon and Temperate Cereals and Grasses Using Bacterial Artificial Chromosome Landing With Fluorescence in Situ Hybridization Genetics, May 1, 2006; 173(1): 349 - 362. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Weiss-Schneeweiss, J. Greilhuber, and G. M. Schneeweiss Genome size evolution in holoparasitic Orobanche (Orobanchaceae) and related genera Am. J. Botany, December 1, 2005; 93(1): 148 - 156. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. G. MURRAY, P. J. DE LANGE, and A. R. FERGUSON Nuclear DNA Variation, Chromosome Numbers and Polyploidy in the Endemic and Indigenous Grass Flora of New Zealand Ann. Bot., December 1, 2005; 96(7): 1293 - 1305. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. J. M. ZONNEVELD, I. J. LEITCH, and M. D. BENNETT First Nuclear DNA Amounts in more than 300 Angiosperms Ann. Bot., August 1, 2005; 96(2): 229 - 244. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. J. LEITCH, D. E. SOLTIS, P. S. SOLTIS, and M. D. BENNETT Evolution of DNA Amounts Across Land Plants (Embryophyta) Ann. Bot., January 1, 2005; 95(1): 207 - 217. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. JOHNSTON, A. E. PEPPER, A. E. HALL, Z. J. CHEN, G. HODNETT, J. DRABEK, R. LOPEZ, and H. J. PRICE Evolution of Genome Size in Brassicaceae Ann. Bot., January 1, 2005; 95(1): 229 - 235. [Abstract] [Full Text] [PDF]
|
|