AOBPreview originally published online on July 20, 2005
Annals of Botany 2005 96(4):683-692; doi:10.1093/aob/mci220
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Expression of Sucrose Synthase Genes Involved in Enhanced Elongation of Pondweed (Potamogeton distinctus) Turions under Anoxia
1 Department of Developmental Biology and Neuroscience, Graduate School of Life Sciences, Tohoku University, Sendai 980-8578, Japan and 2 Laboratory of Bio-adaptation, Graduate School of Agricultural Sciences, Tohoku University, Sendai 981-8555, Japan
* For correspondence. Present address: Miyagi University of Education, Sendai 980-0845, Japan. E-mail kimiharu{at}staff.miyakyo-u.ac.jp
Received: 18 October 2004 Returned for revision: 19 November 2004 Accepted: 31 January 2005 Published electronically: 20 July 2005
• Background and Aims Overwintering buds (turions) of the monocot aquatic pondweed species (Potamogeton distinctus) are highly tolerant to anoxic stress. Sucrose metabolism accompanied by enhanced activity of sucrose synthase (SuSy) operates actively during anaerobic elongation of pondweed turions. The aim of this study is to isolate SuSy genes from the turions and to investigate their transcriptional changes in response to anoxia and other stimuli.
• Methods SuSy genes were isolated from pondweed turions by PCR methods and transcript levels of SuSy genes were examined in response to anoxia, sugars and plant hormones. In addition, the effects of anoxia on SuSy activity were examined both in the soluble fraction and in the microsomal fraction.
• Key Results cDNAs of two SuSy genes (PdSUS1 and PdSUS2) were cloned from pondweed turions. The levels of PdSUS1 transcripts increased under anoxia but did not with sugar treatments. Anoxia-stimulated elongation of turions was further enhanced by 2,4-dichlorophenoxyacetic acid (2,4-D) and suppressed by treatments with sorbitol, 2-deoxyglucose (2-dGlc) and abscisic acid (ABA). The levels of PdSUS1 transcripts were increased by 2,4-D and decreased by sorbitol under anoxia. The levels of PdSUS2 transcripts were not significantly affected by anoxia and any other treatments. SuSy activity of turions under anoxia was enhanced in the soluble fraction, but not in the microsomal fraction.
• Conclusions Up-regulation of PdSUS1 transcription under anoxia may not be attributed to sugar starvation under anoxia. A positive correlation between stem elongation and the level of PdSUS1 transcripts was observed in turions treated with anoxic conditions, 2,4-D and sorbitol. The increase in SuSy activity in the cytosol may contribute to sugar metabolism and sustain stem elongation under anoxia.
Key words: Anaerobic elongation, anoxia, gene expression, plant hormone, pondweed, Potamogeton distinctus, sucrose, sucrose synthase
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