AOBPreview originally published online on July 26, 2005
Annals of Botany 2005 96(5):779-791; doi:10.1093/aob/mci235
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Transmitting Tissue ECM Distribution and Composition, and Pollen Germinability in Sarcandra glabra and Chloranthus japonicus (Chloranthaceae)
Department of Botany, University of Toronto, 25 Willcocks Street, Toronto, Ontario, Canada M5S 3B2
* For correspondence. E-mail tsage{at}botany.utoroonto.ca
Received: 12 January 2005 Returned for revision: 23 March 2005 Accepted: 14 June 2005 Published electronically: 26 July 2005
• Background and Aims Free-flowing surface exudates at the stigmatic (wet versus dry stigma) and adaxial epidermis at the site of angiospermy in carpels of Chloranthaceous species have been proposed to comprise a continuous extracellular matrix (ECM) operating in pollen tube transmission to the ovary. The aim of this research was to establish the spatial distribution and histo/immunochemical composition of the ECM involved in pollen tube growth in Sarcandra glabra and Chloranthus japonicus (Chloranthaceae).
• Methods Following confirmation of the pollen tube pathway, the histo/immunochemical make-up of the ECM was determined with histochemistry on fresh tissue to detect cuticle, esterase, proteins, pectins, and lipids and immunolocalization at the level of the TEM on sections from cryofixed/freeze-substituted tissue to detect molecules recognized by antibodies to homogalacturonans (JIM7, 5), arabinogalactan-proteins (JIM13) and cysteine-rich adhesion (SCA).
• Key Results Pollen germinability is low in both species. When grains germinate, they do so on an ECM comprised of an esterase-positive cuticle proper (dry versus wet stigma). Pollen tubes do not track the surface ECM of stigma or adaxial epidermal cells at the site of angiospermy. Instead, tubes grow between stigmatic cells and subsequently along the inner tangential walls of the stigmatic and adaxial carpel cells at the site of angiospermy. Pollen tubes enter the ovary locule at the base of the funiculus. The stigmatic ECM is distinct by virtue of the presence of anti-JIM5 aggregates, lipids, and a protein recognized by anti-SCA.
• Conclusions The Chloranthaceae joins a growing number of basal angiosperm taxa whereby pollen tubes germinate on a dry versus wet stigma to subsequently grow intercellularly en route to the ovary thereby challenging traditional views that the archetype pollen tube pathway was composed of the surface of stigma and adaxial epidermal cells covered with a free-flowing exudate.
Key words: Chloranthaceae, dry stigma, transmitting tissue, extracellular matrix, homogalacturonans, lipids, anti-SCA
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Lora, J. I. Hormaza, and M. Herrero The progamic phase of an early-divergent angiosperm, Annona cherimola (Annonaceae) Ann. Bot., November 19, 2009; (2009) mcp276v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. L. Sage, K. Hristova-Sarkovski, V. Koehl, J. Lyew, V. Pontieri, P. Bernhardt, P. Weston, S. Bagha, and G. Chiu Transmitting tissue architecture in basal-relictual angiosperms: Implications for transmitting tissue origins Am. J. Botany, January 1, 2009; 96(1): 183 - 206. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. B. Thien, P. Bernhardt, M. S. Devall, Z.-d. Chen, Y.-b. Luo, J.-H. Fan, L.-C. Yuan, and J. H. Williams Pollination biology of basal angiosperms (ANITA grade) Am. J. Botany, January 1, 2009; 96(1): 166 - 182. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Williams Amborella trichopoda (Amborellaceae) and the evolutionary developmental origins of the angiosperm progamic phase Am. J. Botany, January 1, 2009; 96(1): 144 - 165. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Lyew, Z. Li, Y. Liang-Chen, L. Yi-bo, and T. L. Sage Pollen tube growth in association with a dry-type stigmatic transmitting tissue and extragynoecial compitum in the basal angiosperm Kadsura longipedunculata (Schisandraceae) Am. J. Botany, July 1, 2007; 94(7): 1170 - 1182. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Gao, T. L. Sage, and K. W. Osteryoung FZL, an FZO-like protein in plants, is a determinant of thylakoid and chloroplast morphology PNAS, April 25, 2006; 103(17): 6759 - 6764. [Abstract] [Full Text] [PDF]
|
|