AOBPreview originally published online on November 25, 2005
Annals of Botany 2006 97(2):299-304; doi:10.1093/aob/mcj024
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Genetic Variation in Five Mediterranean Populations of Juniperus phoenicea as Revealed by Inter-Simple Sequence Repeat (ISSR) Markers
1 Dipartimento di Biotecnologie e Scienze Molecolari, Università degli Studi dell'Insubria, via J.H. Dunant 3, 21100 Varese, Italy and 2 Dipartimento di Botanica ed Ecologia Vegetale, Via Muroni 25, 07100 Sassari, Italy
* For correspondence. E-mail giorgio.binelli{at}uninsubria.it
Received: 19 May 2005 Returned for revision: 26 July 2005 Accepted: 15 October 2005 Published electronically: 25 November 2005
• Background and Aims The assessment of the genetic variability and the identification of isolated populations within a given species represent important information to plan conservation strategies on a genetic basis. In this work, the genetic variability in five natural populations of Juniperus phoenicea, three from Sardinia, one from Cyprus and the last one in the Maritime Alps was analysed by means of ISSRs, on the hypothesis that the latter could have been a refugial one during the last glaciation.
• Methods ISSRs were chosen because of their ability to detect variation without any prior sequence information. The use of three primers yielded 45 reproducible, polymorphic bands, which were utilized to estimate the basic parameters of genetic variability and diversity.
• Key Results All of the populations analysed harboured an adequate amount of genetic variability, with HS = 0·1299. The proportion of genetic diversity between populations has been estimated by GST = 0·12. The three Sardinian populations are separated, as tested by AMOVA, from the Cyprus and the continental ones.
• Conclusions The results indicate that geographical isolation has represented a major barrier to gene flow in Juniperus phoenicea. This work represents a first step towards a full genetic characterization of a conifer from the Mediterranean, a world biodiversity hotspot confronted with climate change, and thus contributes towards the planning of genetics-informed conservation strategies.
Key words: Juniperus phoenicea L, genetic variation, ISSR, conservation, Cupressaceae
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