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AOBPreview originally published online on May 3, 2007
Annals of Botany 2007 99(6):1213-1222; doi:10.1093/aob/mcm072
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© The Author 2007. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

INVITED REVIEW

Molecular Evidence for a Natural Primary Triple Hybrid in Plants Revealed from Direct Sequencing

Zdenek Kaplan* and Judith Fehrer

Institute of Botany, Academy of Sciences of the Czech Republic, CZ-252 43 Pruhonice, Czech Republic

* For correspondence. E-mail kaplan{at}ibot.cas.cz

Received: 3 January 2007    Returned for revision: 22 January 2007    Accepted: 19 February 2007    Published electronically: 3 May 2007

Background and Aims: Molecular evidence for natural primary hybrids composed of three different plant species is very rarely reported. An investigation was therefore carried out into the origin and a possible scenario for the rise of a sterile plant clone showing a combination of diagnostic morphological features of three separate, well-defined Potamogeton species.

Methods: The combination of sequences from maternally inherited cytoplasmic (rpl20-rps12) and biparentally inherited nuclear ribosomal DNA (ITS) was used to identify the exact identity of the putative triple hybrid.

Key Results: Direct sequencing showed ITS variants of three parental taxa, P. gramineus, P. lucens and P. perfoliatus, whereas chloroplast DNA identified P. perfoliatus as the female parent. A scenario for the rise of the triple hybrid through a fertile binary hybrid P. gramineus x P. lucens crossed with P. perfoliatus is described.

Conclusions: Even though the triple hybrid is sterile, it possesses an efficient strategy for its existence and became locally successful even in the parental environment, perhaps as a result of heterosis. The population investigated is the only one known of this hybrid, P. x torssanderi, worldwide. Isozyme analysis indicated the colony to be genetically uniform. The plants studied represented a single clone that seems to have persisted at this site for a long time.

Key words: Triple hybrid, interspecific hybridization, Potamogeton, Potamogetonaceae, internal transcribed spacer, reproductive isolation, clonal propagation, asexual reproduction


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