AOBPreview published online on August 5, 2002
Annals of Botany, doi:10.1093/aob/mcf194
© 2002 by Annals of Botany Company
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Submitted on November 13, 2002
Affiliation of the authors:
1 Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia, Camino de Vera s/n. 46022 Valencia, Spain;
2 Jardín Botánico, Universidad de Valencia, Calle Quart 80, 46008 Valencia, Spain
* To whom correspondence should be addressed. E-mail: Monica. Boscaiu{at}uv.es.
Flow cytometry, using propidium iodide and 4',6-diamidano-2-phenylindole staining, was used to estimate the nuclear DNA content (2C) and the proportion of A
Revised on March 6, 2002
Accepted on May 30, 2002
Intra- and Interspecific Variation in DNA Content in Cistus (Cistaceae)
PHILIPPE ELLUL1, 
T base pairs in 16 species of the Mediterranean genus Cistus. Genome sizes were shown to be constant within species, since no significant intraspecific variation in 2C DNA content was detected. At the genus level, up to about 1·5-fold differences in absolute DNA amounts were observed, ranging from 3·92 pg in C. crispus to 5·88 pg in C. monspeliensis. The (AT) : (GC) ratio was close to 1, and was similar for all species examined, ranging from 47·87 % AT content in C. clusii, to 50·67 % in C. populifolius. Pink-flowered species (subgenus Cistus) had lower DNA amounts than white-flowered species (subgenera Leucocistus and Halimioides). However, the distribution of DNA amounts in Cistus appeared to be continuous and did not permit a clear separation of infra-generic ranks in the genus.
Key words: Flow cytometry, nuclear DNA content, genome size, A
T content, Cistus, Cistaceae.
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